Difference between revisions of "Part:BBa K525512:Design"
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* Anderson promoter <partinfo>J23110</partinfo>, RBS <partinfo>B0034</partinfo>, BisdA <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> from parts.igem | * Anderson promoter <partinfo>J23110</partinfo>, RBS <partinfo>B0034</partinfo>, BisdA <partinfo>K123000</partinfo> and <partinfo>K123001</partinfo> from parts.igem | ||
| + | |||
| + | * All subparts can be found on one of the kit plates | ||
Revision as of 08:56, 18 September 2011
Polycistronic expression of BisdA and BisdB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 896
Illegal BamHI site found at 1284 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 65
Illegal NgoMIV site found at 421
Illegal AgeI site found at 383
Illegal AgeI site found at 1693 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 256
Illegal BsaI site found at 292
Illegal BsaI site found at 1503
Design Notes
- Polycistronic expression of BisdA and BisdB.
- Sequence of bisdA and bisdB was not entered correctly into the parts.igem. For further information see our notes in BBa_K123000:Experience and BBa_K123001:Experience
Source
- Organism: bisdA and bisdB originated in Sphingomonas bisphenolicum AO1
- DNA (probably) synthesized and with optimated codon-usage for E. coli
- Anderson promoter BBa_J23110, RBS BBa_B0034, BisdA BBa_K123000 and BBa_K123001 from parts.igem
- All subparts can be found on one of the kit plates
References
Sasaki M, Tsuchido T, Matsumura Y (2008) Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1, [http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03843.x/full J Appl Microbiol 105(4):1158-1169].