Difference between revisions of "Part:BBa K123000:Experience"
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'''Wrong sequence in the parts.igem!''' | '''Wrong sequence in the parts.igem!''' | ||
Sequence is not entered into the parts.igem correctly. This BioBrick was probably synthesized in the Freiburg assembly standard 25 because it has the accordant restriction sites and it was codon optimized for ''E. coli'' but the original sequence from ''Sphingomonas bisphenolicum'' was sent to the registry. | Sequence is not entered into the parts.igem correctly. This BioBrick was probably synthesized in the Freiburg assembly standard 25 because it has the accordant restriction sites and it was codon optimized for ''E. coli'' but the original sequence from ''Sphingomonas bisphenolicum'' was sent to the registry. | ||
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+ | [[Image:Bielefeld2011_K123000_DNA.jpg|300px|thumb|left|'''Fig. 1: Alignment of the DNA sequence of <partinfo>K123000</partinfo> entered into the parts.igem (K123000 registry) with our sequencing results (K123000 real) for this BioBrick (made with Clonemanager). ''']] [[Image:Bielefeld2011_K123000_AA.jpg|300px|thumb|right|'''Fig. 2: Alignment of the aminoacid sequence (translated ''in silico'') of <partinfo>K123000</partinfo> entered into the parts.igem (K123000 registry) with our sequencing results (K123000 real) for this BioBrick (made with Clonemanager).''']] | ||
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Revision as of 18:32, 24 August 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K123000
User Reviews
UNIQb174e8cf14d23e48-partinfo-00000000-QINU UNIQb174e8cf14d23e48-partinfo-00000001-QINU
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Bielefeld-Germany 2011 |
Wrong sequence in the parts.igem! Sequence is not entered into the parts.igem correctly. This BioBrick was probably synthesized in the Freiburg assembly standard 25 because it has the accordant restriction sites and it was codon optimized for E. coli but the original sequence from Sphingomonas bisphenolicum was sent to the registry.
Error creating thumbnail: Invalid thumbnail parameters We also carried out these cultivations at different temperatures and BPA concentrations, but the chosen conditions (30 °C and 120 mg L-1 BPA) seem to be the best. Higher BPA concentrations have an effect on the growth of E. coli and higher temperature leeds to a worse BPA degradation (probably due to misfolding of the enzymes). |