Difference between revisions of "Template:RBSMeasurement"
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{{BioCommons}} | {{BioCommons}} | ||
==RBS measurement device for {{{name}}}== | ==RBS measurement device for {{{name}}}== | ||
| − | This device was used to determine {{{name}}} strength relative to <partinfo>B0034</partinfo> | + | This device was used to determine {{{name}}} strength relative to <partinfo>B0034</partinfo>. |
===Authors=== | ===Authors=== | ||
| Line 15: | Line 15: | ||
===Construct design=== | ===Construct design=== | ||
| − | The device consists of <partinfo>J23100</partinfo> promoter followed by [https://parts.igem.org/wiki/index.php/Part:{{{name}}} {{{name}}}] {{{kind|community}}} RBS and <partinfo>I13401</partinfo> reporter module. | + | The device consists of <partinfo>J23100</partinfo> promoter followed by [https://parts.igem.org/wiki/index.php/Part:{{{name}}} {{{name}}}] {{{kind|community}}} RBS and <partinfo>I13401</partinfo> reporter module. |
| + | GFPmut3b was used as reporter protein. | ||
| + | |||
| + | ===Measurements=== | ||
| + | Using this construct we have determined that {{{name}}} strength is '''{{{strength}}}''' relative to <partinfo>B0034</partinfo> | ||
| + | |||
| + | All our measurement results are summarized on the chart below: | ||
| + | |||
| + | https://static.igem.org/mediawiki/2010/e/e9/RBS_relative_strength.png | ||
| + | |||
| + | For more info please look at Team Warsaw 2010 [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS measurement page]. | ||
Revision as of 22:01, 25 October 2010
This part is licensed under
Creative BioCommons
RBS measurement device for {{{name}}}
This device was used to determine {{{name}}} strength relative to BBa_B0034.
Authors
Clonning:
- Ania Puławska
- Ania Safray
- Milena Bażlekowa
The lab work was supervised by:
- Jarek Pankowski
- Ania Olchowik
- Kasia Grześ
Flow cytometer measurements done by Cherry Moreno
Construct design
The device consists of BBa_J23100 promoter followed by {{{name}}} community RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein.
Measurements
Using this construct we have determined that {{{name}}} strength is {{{strength}}} relative to BBa_B0034
All our measurement results are summarized on the chart below:
For more info please look at Team Warsaw 2010 [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS measurement page].