Difference between revisions of "Part:BBa K4244062"
| Line 3: | Line 3: | ||
<partinfo>BBa_K4244062 short</partinfo> | <partinfo>BBa_K4244062 short</partinfo> | ||
| − | + | sfGFP <partinfo>BBa_K4244004</partinfo> was fused to SEC-N22 <partinfo>BBa_K2895007</partinfo> to secrete sfGFP and use it as an extracellular reporter. It is under an constitutive medium strength promoter <partinfo>BBa_K4244057</partinfo>. | |
| + | |||
| + | ====Secretion assay==== | ||
| + | |||
| + | [[File:BBa K4244062-SECN22 ts.png|thumb|500px|center|<font size="1">Figure 1: Time series measurement of total, pellet and supernatant fraction of sfGFP under the SEC-N22 signal peptide expressed with constitutive promoter. Measurement was performed every two hours for eight hours. No significant fluorescence was detected in the supernatant fraction.</font>]] | ||
| + | |||
| + | ====Discussion==== | ||
| + | No secretion was observed in ''E. coli'' Nissle. This may be due to the too high expression level of sfGFP under <partinfo>BBa_K4244057</partinfo>. This experiment still need adjustment and fine tuning to prove the secretion of sfGFP. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 11:33, 10 October 2022
Constitutive expression of sfGFP with SEC-N22 secretion tag
sfGFP BBa_K4244004 was fused to SEC-N22 BBa_K2895007 to secrete sfGFP and use it as an extracellular reporter. It is under an constitutive medium strength promoter BBa_K4244057.
Secretion assay
Discussion
No secretion was observed in E. coli Nissle. This may be due to the too high expression level of sfGFP under BBa_K4244057. This experiment still need adjustment and fine tuning to prove the secretion of sfGFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]