Part:BBa_K2895007
Signal Peptide
Used to bypass the inner membrane of E.coli
Team Colourectal 2022 (Wageningen_UR)
Arthur Vancolen
- This contribution adds useful information to understand how the signal peptide works.
The SEC-N22 signal peptide originates from the curli secretion pathway (Type VIII) (1). This pathway is natively used in E. coli to secrete monomeric protein units of curli fibres (CsgA) which self-assemble into membrane-bound extracellular curli fibres during biofilm formation (2,3). This pathway is composed of seven curli-specific genes (csg) encoded by two operons (csgBAC and csgDEFG). These operons encode the structural subunit of the curli protein and other accessory proteins required for CsgA secretion and assembly (4). CsgA is secreted into the periplasm with the help of the SEC (1-60) peptide and across the outer membrane with the help of the N22 peptide(61-126) (4). These two components are essential for the secretion of proteins through this pathway and are not specific to CsgA. SEC-N22 can thus be fused at the N-termini of your gene of interest for the protein to be secreted extracellularly.
References
1. Bahl F, Chou DB. Engineered E. coli Nissle 1917 for the delivery of matrix-tethered therapeutic domains to the gut. Nat Commun [Internet]. 2019;(2019). Available from: http://dx.doi.org/10.1038/s41467-019-13336-6
2. Kan A, Birnbaum DP, Praveschotinunt P, Joshi NS. Congo red fluorescence for rapid in situ characterization of synthetic curli systems. Appl Environ Microbiol. 2019;85(13):1–13.
3. Blanco LP, Evans ML, Smith DR, Badtke MP, Matthew R. Diversity, biogenesis and function of microbial amyloids. 2013;20(2):66–73.
4. Barnhart MM, Chapman MR. Curli Biogenesis and Function. 2010;131–47.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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