Difference between revisions of "Part:BBa K4115039"
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This composite part is used to detect the response of LuxR [https://parts.igem.org/Part:BBa_C0062 (BBa_C0062)] to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (BBa_R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express. | This composite part is used to detect the response of LuxR [https://parts.igem.org/Part:BBa_C0062 (BBa_C0062)] to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR [https://parts.igem.org/Part:BBa_R0062 (BBa_R0062)] to make superfolder GFP [https://parts.igem.org/Part:BBa_K4115000 (BBa_K4115000)] express. | ||
− | This part is togther used with LuxI [https://parts.igem.org/Part:BBa_C0161 (BBa_C0161)], an AHL(3OC6 HSL here) sender.We can transform the plasmid into the <i>A.caulinodans</i> ORS571 to construct communications and regulations between E. | + | This part is togther used with LuxI [https://parts.igem.org/Part:BBa_C0161 (BBa_C0161)], an AHL(3OC6 HSL here) sender.We can transform the plasmid into the <i>A.caulinodans</i> ORS571 to construct communications and regulations system between <i>E. coli</i> and <i>S.elongatus</i> HL7942 through quorum sensing signals. |
==Proof of function== | ==Proof of function== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K4115039 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4115039 SequenceAndFeatures</partinfo> |
Revision as of 06:52, 4 October 2022
J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015
J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015 | |
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Function | Gene regulator |
Use in | E. coli |
RFC standard | RFC 10, RFC 10 compatible |
Backbone | pBBR1 |
Submitted by | ShanghaiTech_China |
This composite part is used to detect the response of LuxR (BBa_C0062) to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (BBa_R0062) to make superfolder GFP (BBa_K4115000) express.
This part is togther used with LuxI (BBa_C0161), an AHL(3OC6 HSL here) sender.We can transform the plasmid into the A.caulinodans ORS571 to construct communications and regulations system between E. coli and S.elongatus HL7942 through quorum sensing signals.
Proof of function
We encapsulated E. coli with this composite part and E. coli with LuxI into alginate gel beads. The E. coli with LuxI secreted the 3OC6 HSL signal molecule, and the E. coli with LuxR received the signal and regulate the expression of downstream sfGFP, making it express. So we can detect green fluorescence in the latter E. coli under the irradiation of ultraviolet light.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985