Difference between revisions of "Part:BBa K4115039"
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==Proof of function== | ==Proof of function== | ||
| − | [[File:LuxR regulations between E.coli.jpg|500px|thumb|]] | + | [[File:LuxR regulations between E.coli.jpg|500px|thumb|left|]] |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 09:00, 3 October 2022
J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015
| J23101-B0034-luxR-B0015-Lux pR-B0034-sfGFP-B0015 | |
|---|---|
| Function | gene regulator |
| Use in | E.coli |
| RFC standard | RFC 10, RFC 10 compatible |
| Backbone | pBBR1 |
| Submitted by | (ShanghaiTech_China) |
This composite part is used to detect the response of LuxR (C0062) to AHL signaling molecules. When LuxR receives the signal of AHL, it can regulate the downstream Lux pR (R0062) to make superfolder GFP (BBa_K4115000) express.
Then we can transform the plasmid into the A.caulinodans ORS571 to construct communications and regulations between E.coil and S.elongatus through quorum sensing signals.
Proof of function
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985