Difference between revisions of "Part:BBa K3740021"
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<partinfo>BBa_K3740021 parameters</partinfo>
 
<partinfo>BBa_K3740021 parameters</partinfo>
 
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=2021 SZPT-China=
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<h3>Biology</h3>
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<p>The gene rocR is originated from the genome of <i>Pseudomonas aeruginosa</i> (PAO1). And RocR expressed by rocR is the phosphodiesterase (PDE) of c-di-GMP.</p>
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<h3>Usage</h3>
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<p>The encoding sequences for RocR were inserted into the expression vector with <partinfo>BBa_K880005</partinfo> (<partinfo>BBa_J23100</partinfo> & <partinfo>BBa_B0034</partinfo>) to obtain J23100-B0034-rocR-rrnB T1 (<partinfo>BBa_K3740065</partinfo>). We introduced the constructed plasmid into <i>E. coli</i> DH5α to verify its successful construction, and then transferred it into <i>G. hansenii</i> ATCC 53582 to verify its function.
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[[File:szpt15.png|400px|thumb|center|Figure 1. Gene circuit of rocR]]
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<h3>Characterization</h3>
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<h4>1. Identification</h4>
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<p>As shown in Figure 2, c-di-GMP phosphodiesterase-encoded genes <partinfo>BBa_K3740065</partinfo> was identified successfully by PCR amplification.</p>

Revision as of 14:12, 18 October 2021


rocR, PA3947, cyclic di-GMP phosphodiesterase

Description

Degradation of c-di-GMP in Gluconacetobacter hansenii ATCC 53582.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 258
    Illegal BamHI site found at 1171
    Illegal XhoI site found at 94
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 895
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 802


2021 SZPT-China

Biology

The gene rocR is originated from the genome of Pseudomonas aeruginosa (PAO1). And RocR expressed by rocR is the phosphodiesterase (PDE) of c-di-GMP.

Usage

The encoding sequences for RocR were inserted into the expression vector with BBa_K880005 (BBa_J23100 & BBa_B0034) to obtain J23100-B0034-rocR-rrnB T1 (BBa_K3740065). We introduced the constructed plasmid into E. coli DH5α to verify its successful construction, and then transferred it into G. hansenii ATCC 53582 to verify its function.

Figure 1. Gene circuit of rocR

Characterization

1. Identification

<p>As shown in Figure 2, c-di-GMP phosphodiesterase-encoded genes BBa_K3740065 was identified successfully by PCR amplification.