Difference between revisions of "Part:BBa K3740022"
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<p>The encoding sequences for FcsR were inserted into the expression vector with <partinfo>BBa_K880005</partinfo> | <p>The encoding sequences for FcsR were inserted into the expression vector with <partinfo>BBa_K880005</partinfo> | ||
(<partinfo>BBa_J23100</partinfo> & <partinfo>BBa_B0034</partinfo>) to obtain J23100-B0034-fcsR-rrnB T1 (<partinfo>BBa_K3740062</partinfo>). We introduced the constructed plasmid into <i>E. coli</i> DH5α to verify its successful construction, and then transferred it into <i>G. hansenii</i> ATCC 53582 to verify its function.</p> | (<partinfo>BBa_J23100</partinfo> & <partinfo>BBa_B0034</partinfo>) to obtain J23100-B0034-fcsR-rrnB T1 (<partinfo>BBa_K3740062</partinfo>). We introduced the constructed plasmid into <i>E. coli</i> DH5α to verify its successful construction, and then transferred it into <i>G. hansenii</i> ATCC 53582 to verify its function.</p> | ||
| + | [[File:szpt12.png|400px|thumb|center|Figure 1. Gene circuit of fcsR.]] | ||
| + | <h3>Characterization</h3> | ||
| + | <h4>1. Identification</h4> | ||
Revision as of 13:48, 18 October 2021
fcsR, PA2133, cyclic di-GMP phosphodiesterase
Description
Degradation of c-di-GMP in Gluconacetobacter hansenii ATCC 53582.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 703
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 61
Illegal NgoMIV site found at 806 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 700
Illegal BsaI.rc site found at 448
Illegal SapI.rc site found at 813
2021 SZPT-China
Biology
fcsR is originated from the genome of Pseudomonas aeruginosa (PAO1), the FcsR expressed by fcsR is the phosphodiesterase (PDE) of c-di-GMP.
Usage
The encoding sequences for FcsR were inserted into the expression vector with BBa_K880005 (BBa_J23100 & BBa_B0034) to obtain J23100-B0034-fcsR-rrnB T1 (BBa_K3740062). We introduced the constructed plasmid into E. coli DH5α to verify its successful construction, and then transferred it into G. hansenii ATCC 53582 to verify its function.
