Difference between revisions of "Part:BBa K3893026"
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<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] strong ribosome biding site in its Golden Braid compatible version <html></li>
 
<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] strong ribosome biding site in its Golden Braid compatible version <html></li>
  
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<li></html> [https://parts.igem.org/Part:BBa_K2656019 BBa_K2656019]: the [https://parts.igem.org/Part:BBa_I746916 BBa_I746916]  luxI sequence in its Golden Braid standardized version <html></li>
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<li></html> [https://parts.igem.org/Part:BBa_K2656019 BBa_K2656019]: the [https://parts.igem.org/Part:BBa_K2656013 BBa_K2656013]  luxI sequence in its Golden Braid standardized version <html></li>
  
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
 
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
 
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Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence [https://parts.igem.org/Part:BBa_C0161 BBa_C0161] adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon optimized for E. coli.
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Revision as of 19:35, 15 October 2021


luxI constitutive expression Constitutive expressed transcriptional unit assembled with a one-pot Level 1 Golden Gate reaction using BsaI type IIS endonuclease.

This composite part is part of a bigger part to assemble the release system of our Agrobactory 593. It is used to constitutively express LuxI protein, the AHL synthase, fundamental piece of our population oscilator.

This transcriptional unit is composed of the following standardized parts from Valencia_UPV 2018 iGEM Team [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

Part BBa_K2656013 is the Acyl-homoserine-lactone synthase (LuxI) coding sequence BBa_C0161 adapted to the Golden Braid grammar and so used with both Biobrick and Golden Gate assembly methods. It was codon optimized for E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]