Difference between revisions of "Part:BBa K3457003"
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<h2><b>Group: QHFZ-China iGEM 2020</b></h2>
 
<h2><b>Group: QHFZ-China iGEM 2020</b></h2>
 
<p>This part is a basic part. QHFZ-China 2020 measured it in their composite parts, [https://parts.igem.org/Part:BBa_K3457032 K3457032], [https://parts.igem.org/Part:BBa_K3457033 K3457033], [https://parts.igem.org/Part:BBa_K3457034 K3457034], [https://parts.igem.org/Part:BBa_K3457035 K3457035], [https://parts.igem.org/Part:BBa_K3457036 K3457036], [https://parts.igem.org/Part:BBa_K3457037 K3457037], [https://parts.igem.org/Part:BBa_K3457039 K3457039], [https://parts.igem.org/Part:BBa_K3457042 K3457042], [https://parts.igem.org/Part:BBa_K3457043 K3457043].</p>
 
<p>This part is a basic part. QHFZ-China 2020 measured it in their composite parts, [https://parts.igem.org/Part:BBa_K3457032 K3457032], [https://parts.igem.org/Part:BBa_K3457033 K3457033], [https://parts.igem.org/Part:BBa_K3457034 K3457034], [https://parts.igem.org/Part:BBa_K3457035 K3457035], [https://parts.igem.org/Part:BBa_K3457036 K3457036], [https://parts.igem.org/Part:BBa_K3457037 K3457037], [https://parts.igem.org/Part:BBa_K3457039 K3457039], [https://parts.igem.org/Part:BBa_K3457042 K3457042], [https://parts.igem.org/Part:BBa_K3457043 K3457043].</p>
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=== '''Contribution from iBowu-China 2021''' ===
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<p>
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In iGEM 2021, Team iBowu-China adopted T7 promoter to use in combination with LacO and gusA sequence from E. coli and other sources to express an enzyme &beta;-glucuronidase. In our design, we tested the use of this "short" T7 promoter and noted the difference with a complete T7 promoter [1].
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</p>
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[[File:T--iBowu-China--2021bG-3.png|thumb|center|600px|'''Figure.1. The plasmid constructions used for the expression of this part. This part encodes an enzyme &beta;-glucuronidase.''' ]]
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<br/>
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== '''References''' ==
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[1] Jones, J., Vernacchio, V., Lachance, D. et al. ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep 5, 11301 (2015). https://doi.org/10.1038/srep11301
  
  

Revision as of 01:17, 20 October 2021


T7 promoter

T7 promoter of pet-modification vector

Contribution

Group: QHFZ-China iGEM 2020

This part is a basic part. QHFZ-China 2020 measured it in their composite parts, K3457032, K3457033, K3457034, K3457035, K3457036, K3457037, K3457039, K3457042, K3457043.

Contribution from iBowu-China 2021

In iGEM 2021, Team iBowu-China adopted T7 promoter to use in combination with LacO and gusA sequence from E. coli and other sources to express an enzyme β-glucuronidase. In our design, we tested the use of this "short" T7 promoter and noted the difference with a complete T7 promoter [1].

Figure.1. The plasmid constructions used for the expression of this part. This part encodes an enzyme β-glucuronidase.


References

[1] Jones, J., Vernacchio, V., Lachance, D. et al. ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep 5, 11301 (2015). https://doi.org/10.1038/srep11301




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]