Difference between revisions of "Part:BBa K3165037"

Revision as of 01:52, 21 October 2019

mini-CcaS Generator This part is used as a generator for mini-CcaS (BBa_K3165000) under a constitutive promoter (BBa_J23106) and a strong ribosome site (BBa_K3165005) with a double terminator (BBa_B0015) .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain.
It is the light-sensitive part of the photo-sensitive CcaS-CcaR system. This part helps in the synthesis of CcaS protein constitutively under a strong ribosome binding site. In association with the Ho1-PcyA-CcaR gene system, this part forms the complete optogenetic CcaS-CcaR system.

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 __NOTOC__
-<partinfo>BBa_K3165037 short</partinfo>K
+<partinfo>BBa_K3165037 short</partinfo>
 This part is used as a generator for mini-CcaS <html><a  href="https://parts.igem.org/Part:BBa_K3165000">(BBa_K3165000)</a></html> under a constitutive promoter <html><a  href="https://parts.igem.org/Part:BBa_J23106">(BBa_J23106)</a></html> and a strong ribosome site <html><a  href="https://parts.igem.org/Part:BBa_K3165005">(BBa_K3165005)</a></html> with a double terminator <html><a  href="https://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a></html> .