Difference between revisions of "Help:Plasmid backbones"
| Line 1: | Line 1: | ||
| − | + | [[Image:BioBrickvectorwithpart.png]] | |
| − | + | ||
| − | [[ | + | |
| − | + | ||
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell] independently of the chromosomal DNA. Plasmid DNA is easily purified from cells, manipulated using common lab techniques and incorporated into cells. Most BioBrick parts in the Registry are maintained and propagated on plasmids. Thus, construction of BioBrick parts, devices and systems usually requires working with plasmids. | [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell] independently of the chromosomal DNA. Plasmid DNA is easily purified from cells, manipulated using common lab techniques and incorporated into cells. Most BioBrick parts in the Registry are maintained and propagated on plasmids. Thus, construction of BioBrick parts, devices and systems usually requires working with plasmids. | ||
| Line 18: | Line 16: | ||
*'''[[Plasmids|Browse available plasmids]]''' [[Image:Part icon plasmid.png]] | *'''[[Plasmids|Browse available plasmids]]''' [[Image:Part icon plasmid.png]] | ||
| − | |||
Revision as of 00:21, 5 September 2008
Plasmids are circular, double-stranded DNA molecules typically containing a few thousand base pairs that replicate within the cell independently of the chromosomal DNA. Plasmid DNA is easily purified from cells, manipulated using common lab techniques and incorporated into cells. Most BioBrick parts in the Registry are maintained and propagated on plasmids. Thus, construction of BioBrick parts, devices and systems usually requires working with plasmids.
- Glossary - What are plasmids, vectors and backbones?
- Plasmid features - Common features of plasmids
- Plasmid naming - How BioBrick vectors are named
- Finding plasmids - How to locate plasmids in the Registry
- Constructing new plasmids - How to construct new BioBrick plasmids
- Entering new plasmids in the Registry - How to enter new plasmid backbones in the Registry
