Difference between revisions of "Part:BBa K2656012"
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__NOTOC__
 
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<partinfo>BBa_K2656012 short</partinfo>
 
<partinfo>BBa_K2656012 short</partinfo>
  
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Part BBa_K2656012 is the RBS [https://parts.igem.org/Part:BBa_J61101 BBa_J61101] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 GoldenBraid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
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BBa_K2656012 is the [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] ribosome binding site standardized into the Golden Braid assembly method. Thus, it is strong RBS compatible both with the BioBrick and Golden Gate grammar.
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Using the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other GB adapted basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a single one-pot reaction.
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Characterization of the this part was performed with the transcriptional unit [https://parts.igem.org/Part:BBa_K2656104 BBa_K2656104], which was used in a comparative RBS expression experiment with composite parts [https://parts.igem.org/Part:BBa_K2656100 BBa_K265610 ] and [https://parts.igem.org/Part:BBa_K2656101 BBa_K2656101].
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They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator:
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<ul>
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<li></html>Promoter [https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
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<li></html>CDS [https://parts.igem.org/Part:BBa_K2656013 BBa_K2656013]: the [https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009] sfGFP sequence in its Golden Braid standardized version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
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<li></html>Terminator [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
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</ul>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2656012 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2656008 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2656012 parameters</partinfo>
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<partinfo>BBa_K2656008 parameters</partinfo>
 
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Revision as of 16:18, 12 October 2018

Very Low Ribosome Binding Site J61101

BBa_K2656012 is the BBa_B0030 ribosome binding site standardized into the Golden Braid assembly method. Thus, it is strong RBS compatible both with the BioBrick and Golden Gate grammar.

Using the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol], it can be combined with other GB adapted basic parts, such as the ones from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection], to assemble transcriptional units in a single one-pot reaction.

Characterization of the this part was performed with the transcriptional unit BBa_K2656104, which was used in a comparative RBS expression experiment with composite parts BBa_K265610 and BBa_K2656101. They all were assembled in a Golden Braid alpha1 plasmid using the same promoter, CDS and terminator:

  • Promoter BBa_K2656004: the J23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • CDS BBa_K2656013: the BBa_K2656009 sfGFP sequence in its Golden Braid standardized version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • Terminator BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]