Difference between revisions of "Part:BBa K2623016"
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===SUMMARY===
 
===SUMMARY===
 
TDP circuit is used to express exocrine protein SAHS.
 
TDP circuit is used to express exocrine protein SAHS.
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We added amilCP <partinfo>BBa_K592009</partinfo> as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter <partinfo>BBa_J23100</partinfo> and an RBS <partinfo>BBa_B0034</partinfo>, and followed by a double terminator <partinfo>BBa_B0015</partinfo>.
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We added amilCP <partinfo>BBa_K592009</partinfo> as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter <partinfo>BBa_J23100</partinfo> and an RBS <partinfo>BBa_B0034</partinfo>, and followed by a double terminator <partinfo>BBa_B0015</partinfo>.<br>
  
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https://static.igem.org/mediawiki/parts/4/4e/CAHS_Fig1.png
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 15:00, 1 October 2018


Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator)

SUMMARY

TDP circuit is used to express exocrine protein SAHS. We added amilCP BBa_K592009 as a reporter gene to the gene circle for us to screen and validate. Because our aim is to obtain a large number of SAHS proteins, the constant promoter J23100 is chosen to allow SAHS to be expressed continuously. The sequence encoding for the gene is preceded by a constant promoter BBa_J23100 and an RBS BBa_B0034, and followed by a double terminator BBa_B0015.

CAHS_Fig1.png Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 279
    Illegal XhoI site found at 503
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 558
    Illegal SapI.rc site found at 553