Difference between revisions of "Part:BBa K2656018"
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<partinfo>BBa_K2656018 short</partinfo>
 
<partinfo>BBa_K2656018 short</partinfo>
  
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Part BBa_K592009 standarized as Phytobrick. Blue chromoprotein. It can be used to assemble transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.
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Part BBa_K2656021 is the Yellow Fluorescent Protein coding secuence [https://parts.igem.org/Part:BBa_K592101 BBa_K592101] compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Braid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].
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The characterization of this protein (and by extension of all the other part that codify for the sfGFP) has been performed with our transcriptional unit [https://parts.igem.org/Part:BBa_K2656113 BBa_K2656113].
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This transcriptional unit was assembled in a Golden Braid alpha1 plasmid'''(REF)''' including the following parts:
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<html>
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<ul>
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<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656009 BBa_K2656009]: the [https://parts.igem.org/Part:BBa_B0030 B0030] ribosome biding site in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656018 BBa_K2656018]: This part.<html></li>
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<li></html>[https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]<html></li>
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</ul>
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</html>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:14, 30 September 2018


amilCP Coding Sequence

Part BBa_K2656021 is the Yellow Fluorescent Protein coding secuence BBa_K592101 compatible with both Biobrick and [http://2018.igem.org/Team:Valencia_UPV/GB3 Golden Braid 3.0] assemply methods. It can be combined with other compatible parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Valencia UPV IGEM 2018 Printeria Collection] to assemble transcriptional units with the [http://2018.igem.org/Team:Valencia_UPV/Protocols Golden Gate assembly protocol].

The characterization of this protein (and by extension of all the other part that codify for the sfGFP) has been performed with our transcriptional unit BBa_K2656113. This transcriptional unit was assembled in a Golden Braid alpha1 plasmid(REF) including the following parts:

  • BBa_K2656004: the J23106 promoter in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • BBa_K2656009: the B0030 ribosome biding site in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]
  • BBa_K2656018: This part.
  • BBa_K2656026: the B0015 transcriptional terminator in its Golden Braid compatible version from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]