Difference between revisions of "Part:BBa I739003"

Revision as of 12:46, 19 October 2007

Constitutive expression cassette for LuxR (J23100.B0034.C0062.B0015)

Part Structure

The Biobrick encodes LuxR (BBa_C0062) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of luxR is terminated by the double terminator BBa_B0015.

Mode of Action

Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL) (e.g. AHL). This complex binds to a palindromic site on the promoter BBa_R0062, increasing the rate of transcription. So far, this LuxI/R system is the best characterized system of all cell-cell signaling systems.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed Part 3. The constitutively synthesized luxR interacts with .... when complexed with HSL.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features