Difference between revisions of "Part:BBa I739002"
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===Mode of Action=== | ===Mode of Action=== | ||
− | <p>LacI binds to the pLac regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0010 BBa_R0010)] (or to the PLlac01 hybrid regulator [https://parts.igem.org/wiki/index.php/Part:BBa_R0011 BBa_R0011] respectively and represses transcription. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed.</p> | + | <p>LacI binds to the pLac regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0010 BBa_R0010)] (or to the PLlac01 hybrid regulator [https://parts.igem.org/wiki/index.php/Part:BBa_R0011 BBa_R0011] respectively) and represses transcription. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed.</p> |
===Purpose=== | ===Purpose=== |
Revision as of 12:09, 11 October 2007
Constitutive expression cassette for LacI +LVA (J23100.B0034.C0012.B0015)
Part Structure
The Biobrick encodes LVA-tagged lacI (BBa_C0012) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of lacI is terminated by the double terminator BBa_B0015.
Mode of Action
LacI binds to the pLac regulator (BBa_R0010) (or to the PLlac01 hybrid regulator BBa_R0011 respectively) and represses transcription. If the inducer [http://openwetware.org/wiki/IPTG Isopropyl-beta-D-thiogalactopyranoside (IPTG)] is added, lacI action is inhibited and the promoter gets derepressed.
Purpose
This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project describtion, this part is also termed Part 1. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is BBa_Q04400.
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1208
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]