Difference between revisions of "Part:BBa I739001"
Line 9: Line 9:
  
 
===Purpose===
 
===Purpose===
−
<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project describtion, this part is also termed '''Part 1'''. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04400 BBa_Q04400].</p>
+
<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project describtion, this part is also termed ''Part 1''. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is [https://parts.igem.org/wiki/index.php/Part:BBa_Q04400 BBa_Q04400].</p>
  
 
===Testing===
 
===Testing===
−
<p>Checked for uniqueness of restriction enzyme cleavage sites:
+
<p>Checked for uniqueness of restriction enzyme cleavage sites:<br>
 
Eco: ok<br>
 
Eco: ok<br>
 
Xba: ok<br>
 
Xba: ok<br>
 
Spe: ok<br>
 
Spe: ok<br>
 
Pst: ok<br>
 
Pst: ok<br>
−
</p><br>
+
</p>
 +
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 11:51, 11 October 2007

Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)

Part Structure

The Biobrick encodes LVA-tagged tetR (BBa_C0040) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015.

Mode of Action

TetR binds to the pTet regulator (BBa_R0040) and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.

Purpose

This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project describtion, this part is also termed Part 1. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is BBa_Q04400.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]