Difference between revisions of "Part:BBa I739001"
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===Part Structure=== | ===Part Structure=== | ||
− | The Biobrick encodes LVA-tagged tetR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0040 BBa_C0040]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100], followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of tetR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].< | + | <p>The Biobrick encodes LVA-tagged tetR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0040 BBa_C0040]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100], followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of tetR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p><br> |
===Mode of Action=== | ===Mode of Action=== | ||
− | TetR binds to the pTet regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0040 BBa_R0040)] and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed. | + | <p>TetR binds to the pTet regulator ([https://parts.igem.org/wiki/index.php/Part:BBa_R0040 BBa_R0040)] and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.</p> |
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Revision as of 20:25, 10 October 2007
Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)
Part Structure
The Biobrick encodes LVA-tagged tetR (BBa_C0040) under control of the constitutive promoter BBa_J23100, followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015.
Mode of Action
TetR binds to the pTet regulator (BBa_R0040) and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]