Difference between revisions of "Part:BBa J45017"
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<partinfo>BBa_J45017 short</partinfo>
 
<partinfo>BBa_J45017 short</partinfo>
  
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PchBA codes for proteins used to generate salicylic acid (the precursor to methyl salicylate or wintergreen scent) from chorismate, a common metabolite found in most bacteria. PchB and PchA are at the downstream end of the PchDCBA operon that ''Pseudomonas'' species use to encode enzymes to make '''p'''yo'''ch'''elin, a molecule that helps them acquire iron. This part doesn't make a fusion protein, even though the coding regions of the two genes overlap; the RBS for PchA is located at the end of the coding region of PchB.
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<partinfo>BBa_J45017</partinfo> encodes an isochorismate pyruvate-lyase and an isochorismate synthase derived from the ''pchBA'' genes from ''Pseudomonas aeruginosa''.  PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate.  Salicylic acid is the precursor to methyl salicylate (wintergreen odor)
  
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===Usage and Biology===
 
===Usage and Biology===
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''pchB'' and ''pchA'' are at the downstream end of the ''pchDCBA'' operon that ''Pseudomonas'' species use to encode enzymes to make '''p'''yo'''ch'''elin, a molecule that helps them acquire iron.  <partinfo>BBa_J45017</partinfo> doesn't make a fusion protein but the coding regions of the two genes do overlap.  The RBS for ''pchA'' is located at the end of the coding region of ''pchB''.
  
 
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Revision as of 21:27, 7 March 2008

isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate

BBa_J45017 encodes an isochorismate pyruvate-lyase and an isochorismate synthase derived from the pchBA genes from Pseudomonas aeruginosa. PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Salicylic acid is the precursor to methyl salicylate (wintergreen odor)

Usage and Biology

pchB and pchA are at the downstream end of the pchDCBA operon that Pseudomonas species use to encode enzymes to make pyochelin, a molecule that helps them acquire iron. BBa_J45017 doesn't make a fusion protein but the coding regions of the two genes do overlap. The RBS for pchA is located at the end of the coding region of pchB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 711
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 1128
    Illegal NgoMIV site found at 1596
  • 1000
    COMPATIBLE WITH RFC[1000]