Difference between revisions of "Part:BBa J45800"

Revision as of 18:13, 13 March 2008

Exponential phase wintergreen odor biosynthetic system

We assembled a complete, exponential phase dependent wintergreen odor biosynthetic system (BBa_J45800) by combining the salicylic acid (BBa_J45320) and exponential phase dependent wintergreen odor generators (BBa_J45181). BBa_J45800 catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor. Wintergreen odor should only be produced in exponentially growing cells.

Usage and Biology

To construct a complete wintergreen odor biosynthetic system, we needed to design an additional biosynthetic device for wintergreen odor precursor production. We designed a biosynthetic device for production of salicylate from the cellular metabolite chorismate (BBa_J45320) based on the pchBA genes from Pseudomonas aeruginosa. The enzyme PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate. The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate. Serino et al. previously demonstrated salicylate formation in E. coli using the pchBA genes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 3620
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 389
Illegal NgoMIV site found at 793
Illegal NgoMIV site found at 918
Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 1210
Illegal NgoMIV site found at 1678
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 3161

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 <partinfo>BBa_J45800 short</partinfo>
-This system designed by the MIT 2006 iGEM team generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent).  It uses a stationary phase promoter followed by an inverter (see <bbpart>BBa_J45996</bbpart> for details) to only express the necessary enzymes during exponential phase.
+We assembled a complete, exponential phase dependent wintergreen odor biosynthetic system (<partinfo>BBa_J45800</partinfo>) by combining the salicylic acid (<partinfo>BBa_J45320</partinfo>) and exponential phase dependent wintergreen odor generators (<partinfo>BBa_J45181</partinfo>).  <partinfo>BBa_J45800</partinfo> catalyzes the conversion of the cellular metabolite chorismate to methyl salicylate or wintergreen odor.  Wintergreen odor should only be produced in exponentially growing cells.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+To construct a complete wintergreen odor biosynthetic system, we needed to design an additional biosynthetic device for wintergreen odor precursor production.  We designed a biosynthetic device for production of salicylate from the cellular metabolite chorismate (<partinfo>BBa_J45320</partinfo>) based on the ''pchBA'' genes from ''Pseudomonas aeruginosa''.  The enzyme PchA, an isochorismate synthase, catalyzes the conversion of chorismate to isochorismate.  The enzyme PchB, an isochorismate pyruvate-lyase, catalyzes the conversion of isochorismate to salicylate and pyruvate.  Serino ''et al.'' previously demonstrated salicylate formation in ''E. coli'' using the ''pchBA'' genes.
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