Difference between revisions of "Part:BBa K1412614"

Revision as of 14:24, 11 October 2014

Characterize the efficiency of promoter(BBa_K206000) with chemotaxis

BBa_K1412614: pBAD- RBS (1.0)-CheZ-TT

This part consists of a [http://en.wikipedia.org/wiki/Chemotaxis CheZ] gene which can express CheZ protein deciding E.coli whether tumbling or swimming straight. In this light, we can characterize the efficiency of promoter by just replacing different promoters before a CheZ gene. Then we can characterize the efficiency of promoter via measuring the migration distance positively associated with the expression strength of CheZ protein.

Usage

When we want to characterize the efficiency of promoter, we usually link the promoter with GFP, then characterize the efficiency of promoter by measuring the fluorescence intensity of GFP. In our part, you need just link RBS (1.0) after a pBAD promoter and before a CheZ gene, ended with a TT terminator (pBAD-RBS (1.0)-CheZ-TT). Then transfer this gene circuit into E.coli (CheZ knocked out), coat plates, and culture on semi-solid medium to measure the migration diameter of E.coli.

Relevant parts

BBa_K1412000: pLac-RBS(1.0)-CheZ-TT CheZ generator under pLac promoter

BBa_K1412005: RBS(1.0)-CheZ-TT

BBa_K1412006: RBS(0.01)-CheZ-TT

BBa_K1412007: RBS(0.3)-CheZ-TT

BBa_K1412014: pTetR-RBS(1.0)-CheZ-TT Characterize the efficiency of promoters with chemotaxis

BBa_K1412801: pLac-RBS(0.01)-CheZ-TT Characterize the efficiency of RBS with chemotaxis

BBa_K1412829: Plac-RBS(0.3)-CheZ-TT Characterize efficiency of RBS with chemotaxis

Notes

Source

BBa_K206000

BBa_B0034

BBa_K629003

BBa_B0015

Sequence and Features

Reference