Difference between revisions of "Part:BBa K1045017:Design"
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===Source=== | ===Source=== | ||
| − | This composite part was constructed using hybridization oligos for [[Part:BBa_K1045012|BBa_1045012]] and [[Part:BBa_K1045011|BBa_1045011]] or | + | This composite part was constructed using hybridization oligos for [[Part:BBa_K1045012|BBa_1045012]] and [[Part:BBa_K1045011|BBa_1045011]] or parts that were obtained either by amplification of plasmid DNA ([[Part:BBa_K1045009|BBa_1045009]]) or ''M. smegmatis'' chromosomal DNA ([[Part:BBa_K1045001|BBa_1045001]]). Part [[Part:BBa_E0240|BBa_E0240]] derived from the plasmid in the distribution kit. |
===References=== | ===References=== | ||
Revision as of 17:27, 4 October 2013
DarR reporter system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 806
Illegal NheI site found at 829
Illegal NheI site found at 909
Illegal NheI site found at 932
Illegal NotI site found at 718 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 731
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 860
Illegal AgeI site found at 168 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1630
Design Notes
DarR fused to the terminator were cut out from BBa_K1045016 and ligated in a pre-fixing composition into BBa_K1045015.
Source
This composite part was constructed using hybridization oligos for BBa_1045012 and BBa_1045011 or parts that were obtained either by amplification of plasmid DNA (BBa_1045009) or M. smegmatis chromosomal DNA (BBa_1045001). Part BBa_E0240 derived from the plasmid in the distribution kit.