Difference between revisions of "Part:BBa K1073024"

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<partinfo>BBa_K1073024 short</partinfo>
 
<partinfo>BBa_K1073024 short</partinfo>
  
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The device consists of a strong promoter (<partinfo>BBa_J23100</partinfo>) combined with a RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the chromoprotein amilGFP. This results in a constitutively high expression of amilGFP.  
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This device is an enhanced construct of 2012 Uppsala's <partinfo>BBa_K592010</partinfo>. It consists of a strong promoter (<partinfo>BBa_J23100</partinfo>) combined with a RBS (<partinfo>BBa_B0032</partinfo>) and the encoding region of the chromoprotein amilGFP (<partinfo>BBa_K592010</partinfo>). This results in a constitutively high expression of amilGFP.  
  
  

Revision as of 17:57, 4 October 2013

Constitutively expressed chromoprotein amilGFP

This device is an enhanced construct of 2012 Uppsala's BBa_K592010. It consists of a strong promoter (BBa_J23100) combined with a RBS (BBa_B0032) and the encoding region of the chromoprotein amilGFP (BBa_K592010). This results in a constitutively high expression of amilGFP.


Usage and Biology

During cultivation, the chromoprotein amilGFP is expressed and stored intracellularly. The yellow color of the protein can be seen by the naked eye, thus the chromoprotein can be used as a reporter or selection marker to distinguish cells easily.

Expressed chromoproteins.png AmilGFP pellet.png

iGEM Team Braunschweig 2013:

Left: Liquid cultures of E. coli XL1 expressing chromoproteins amilGFP , eforRed (BBa_K1073022) and aeBlue (BBa_K1073020).

Right: Cell pellet of E. coli XL1 expressing amilGFP.


The yellow color of amilGFP can be seen by the naked eye. The chromoprotein also fluoresce when excited. Thus it can be used to identify cells with fluorescence microscopy or flow cytometry. For further information as well as absorption and emission spectra see experience page.


Fluorescence.JPG

iGEM Team Braunschweig 2013: Supernatant after cell disruption containing chromoproteins of eforRed (BBa_K1073022) and amilGFP. Both chromoproteins fluoresce when excited by UV-light.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]