Difference between revisions of "Part:BBa E1010:Experience"
Enpederson (Talk | contribs) (→User Reviews) |
Enpederson (Talk | contribs) (→User Reviews) |
||
Line 10: | Line 10: | ||
===Applications of BBa_E1010=== | ===Applications of BBa_E1010=== | ||
− | ===User Reviews=== | + | [[File:Example.jpg]]===User Reviews=== |
<!-- DON'T DELETE --><partinfo>BBa_E1010 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_E1010 StartReviews</partinfo> | ||
<!-- Template for a user review | <!-- Template for a user review | ||
Line 190: | Line 190: | ||
|- | |- | ||
|width='10%'| | |width='10%'| | ||
− | <partinfo>BBa_E1010 AddReview | + | <partinfo>BBa_E1010 AddReview 5</partinfo> |
<I>Carnegie_Mellon 2013</I><br /> | <I>Carnegie_Mellon 2013</I><br /> | ||
<b> Characterization of the Photostability of mRFP1</b> | <b> Characterization of the Photostability of mRFP1</b> | ||
− | [[Image:MRFP1.png|center|thumb|400px|''Photobleaching curve of mRFP1 with a HBO100 mercury-arc lamp"]] | + | [[Image: MRFP1.png|center|thumb|400px|''Photobleaching curve of mRFP1 with a HBO100 mercury-arc lamp"]] |
XL10 Ultracompetent cells were transformed with <partinfo>E1010</partinfo> with <partinfo>R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 3. Fluorescence values are shown in Table 4. <br /> | XL10 Ultracompetent cells were transformed with <partinfo>E1010</partinfo> with <partinfo>R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 3. Fluorescence values are shown in Table 4. <br /> | ||
<table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%"> | <table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%"> |
Revision as of 17:41, 3 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
RANDOM SEQUENCE FOUND WITHIN PART
CGCTGATAGTGCTAGTGTAGATCGC is found after the RFP stop codon and before the BioBricks suffix. Should not affect transcription or translation of RFP, but good to keep note of it especially in analyzing sequencing results. (KP of siGEM)
- Please note that the above sequence is the old "barcode" sequence added to all of the original CDSs in the early BioBrick part collections. I.e., it's not a random sequence. See https://parts.igem.org/cgi/htdocs/barcodes.cgi for more information (D. Endy).
- FURTHER NOTE The Registry is not displaying barcodes on any of the original parts. The presented sequence information is wrong. This is a serious bug in the Registry that need to be fixed (D. Endy). Drew 14:34, 1 November 2010 (UTC)
Applications of BBa_E1010
===User Reviews=== UNIQ620cadfb2137a81c-partinfo-00000000-QINU
••••
KAIST_iGEM_2012 |
|
•••
DTU_igem_2010 |
Characterization of RFP BBa_E1010 Method Results
|
Antiquity |
This review comes from the old result system and indicates that this part did not work in some test. |
Nkessler |
We successfully used this part for a read out system, e.g. in BBa_K389016. Additionally we compared it with a luciferase: BBa_K389004. |
•••••
Carnegie_Mellon 2013 XL10 Ultracompetent cells were transformed with BBa_E1010 with BBa_R0010 as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 3. Fluorescence values are shown in Table 4.
|
UNIQ620cadfb2137a81c-partinfo-0000000F-QINU