Difference between revisions of "Light"
 
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=Photoproteins=  
 
=Photoproteins=  
 
Aequorin, Obelin
 
Aequorin, Obelin
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=Split-BioBricks for protein interactions=
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=Degradation Tags for kinetic measurements=

Latest revision as of 12:22, 7 March 2013

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Fluorescent Proteins

Featured Parts, [http://www.microscopyu.com/articles/livecellimaging/fpintro.html Nikkon],


Name Exitation [nm] Emmission [nm] Quant. yield Molar extinct. BioBrick RFC10 BioBrick RFC25 Reference
Cerulean 433 475 0.62 43000 BBa_E2020
GFPmut3b 501 511 BBa_E0040
amilGFP 503 512 0.67 75200 BBa_K592010 [http://www.ncbi.nlm.nih.gov/nuccore/ay646067 GeneBank]
mVenus 515 528 0.57 92200 BBa_I757008
mOrange 548 562 0.69 71000 BBa_E2050
mStrawbery 574 596 0.29 90000 BBa_K399001
mRFP1 584 607 0.25 50000 BBa_E1010
mCherry 587 610 0.22 72000 BBa_E2060
eforRed 589 609 0.16 111300 BBa_K592012 [http://www.ncbi.nlm.nih.gov/nuccore/EU498726 GeneBank]
cjBlue 610 BBa_K592011 [http://www.ncbi.nlm.nih.gov/pubmed/17028187 PubMed]
aeBlue BBa_K864401 [http://www.ncbi.nlm.nih.gov/pubmed/16164420 PubMed]
amilCP 588 NA NA 8760 BBa_K592009 [http://www.ncbi.nlm.nih.gov/nuccore/AY646075 GeneBank]


Luciferases

Name Organism Substrate pH optimum Amino acids Mol. mass [kDa] Emmission [nm] BioBrick RFC10 BioBrick RFC25 Reference
Photinus/Firefly luciferase D-luciferin + ATP 550 60.6 578 BBa_I712019
Renilla luciferase coelenterazine + O2 311 36.0 480 BBa_J52008
Gaussia luciferase coelenterazine + O2 189 20.3 480 BBa_K509005
Nano luciferase furimazine 171 19.1 460 [http://www.reportergene.com/2013/01/the-nanoluc-luciferase-reporter-dossier.html 1]

Photoproteins

Aequorin, Obelin

Split-BioBricks for protein interactions

Degradation Tags for kinetic measurements