Difference between revisions of "Part:BBa E0040:Experience"
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− | [[image: KAIST_iGEM_2012_Experience_BBa_E0040.PNG|center|thumb|400px|'''Figure 1. E.coli strain MG1655 expressing BBa_E0040 | + | [[image: KAIST_iGEM_2012_Experience_BBa_E0040.PNG|center|thumb|400px|'''Figure 1. E.coli strain MG1655 expressing BBa_E0040 under control of <partinfo>BBa_K907004</partinfo> after overnight culture in 3mL M9 media with 14 mL round bottom tube(Top left two) and centrifuged cells in eppendorf tube(Bottom left two).''' The expression of BBa_0040 is clearly observed with naked eye after overnight culture.]] |
<br><partinfo>BBa_E0040</partinfo> was successfully used to produce GFPmut3b in E.coli strain MG1655 in LB or M9 minimal media under the control of promoter-<partinfo>BBa_J23119</partinfo> and RBS-<partinfo>BBa_B0034</partinfo> in the Dual Phase Protein Generator(GFP default), <partinfo>BBa_K907004</partinfo>. | <br><partinfo>BBa_E0040</partinfo> was successfully used to produce GFPmut3b in E.coli strain MG1655 in LB or M9 minimal media under the control of promoter-<partinfo>BBa_J23119</partinfo> and RBS-<partinfo>BBa_B0034</partinfo> in the Dual Phase Protein Generator(GFP default), <partinfo>BBa_K907004</partinfo>. | ||
Revision as of 23:28, 26 September 2012
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Applications of BBa_E0040
User Reviews
UNIQe40ec5cedc08ed88-partinfo-00000000-QINU
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KAIST_iGEM_2012 |
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
UNIQe40ec5cedc08ed88-partinfo-00000008-QINU
E0040 contains 2 dpnI sites. This is useful when using a part containing E0040 as a template for PCR because you can cut up the template via a dpnI digest. For this to work the cell strain that the template was purified from must be dam methylase+.
iGEM11_WHU_China |
This part was used by WHU_China team as a reporter of long-term oscillator. Information from the website of this part shows that its length is about 720bp; however, the result of restriction enzymes cleavage experiment showed that it is actually about 1kb. And after several attempts, we failed to assemble this part with other biobricks. |