Difference between revisions of "Part:BBa E0040:Experience"

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[[image: KAIST_iGEM_2012_Experience_BBa_E0040.PNG|center|thumb|400px|'''Figure 1. E.coli strain MG1655 expressing BBa_E0040 in LB under control of <partinfo>BBa_K907004</partinfo> after overnight culture(Left two of top panel & bottom panel).''' The expression of BBa_0040 is clearly observed with naked eye after overnight culture.]]
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[[image: KAIST_iGEM_2012_Experience_BBa_E0040.PNG|center|thumb|400px|'''Figure 1. E.coli strain MG1655 expressing BBa_E0040 under control of <partinfo>BBa_K907004</partinfo> after overnight culture in 3mL M9 media with 14 mL round bottom tube(Top left two) and centrifuged cells in eppendorf tube(Bottom left two).''' The expression of BBa_0040 is clearly observed with naked eye after overnight culture.]]
 
<br><partinfo>BBa_E0040</partinfo> was successfully used to produce GFPmut3b in E.coli strain MG1655 in LB or M9 minimal media under the control of promoter-<partinfo>BBa_J23119</partinfo> and RBS-<partinfo>BBa_B0034</partinfo> in the Dual Phase Protein Generator(GFP default), <partinfo>BBa_K907004</partinfo>.
 
<br><partinfo>BBa_E0040</partinfo> was successfully used to produce GFPmut3b in E.coli strain MG1655 in LB or M9 minimal media under the control of promoter-<partinfo>BBa_J23119</partinfo> and RBS-<partinfo>BBa_B0034</partinfo> in the Dual Phase Protein Generator(GFP default), <partinfo>BBa_K907004</partinfo>.
  

Revision as of 23:28, 26 September 2012

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_E0040

User Reviews

UNIQe40ec5cedc08ed88-partinfo-00000000-QINU

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KAIST_iGEM_2012

Figure 1. E.coli strain MG1655 expressing BBa_E0040 under control of BBa_K907004 after overnight culture in 3mL M9 media with 14 mL round bottom tube(Top left two) and centrifuged cells in eppendorf tube(Bottom left two). The expression of BBa_0040 is clearly observed with naked eye after overnight culture.


BBa_E0040 was successfully used to produce GFPmut3b in E.coli strain MG1655 in LB or M9 minimal media under the control of promoter-BBa_J23119 and RBS-BBa_B0034 in the Dual Phase Protein Generator(GFP default), BBa_K907004.


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Antiquity

This review comes from the old result system and indicates that this part worked in some test.

UNIQe40ec5cedc08ed88-partinfo-00000008-QINU

E0040 contains 2 dpnI sites. This is useful when using a part containing E0040 as a template for PCR because you can cut up the template via a dpnI digest. For this to work the cell strain that the template was purified from must be dam methylase+.

iGEM11_WHU_China

This part was used by WHU_China team as a reporter of long-term oscillator. Information from the website of this part shows that its length is about 720bp; however, the result of restriction enzymes cleavage experiment showed that it is actually about 1kb. And after several attempts, we failed to assemble this part with other biobricks.

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