Difference between revisions of "Part:BBa J23103:Experience"

Revision as of 21:38, 9 November 2014

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Applications of BBa_J23103

Use of this promoter by team Glasgow 2014

BBa_J23116, BBa_J23106, BBa_J23103, and BBa_J23112 were used to express motA and motB together in our composite biobricks:
BBa_ K1463773, BBa_ K1463772, BBa_ K1463770, and BBa_ K1463771 respectively.
These composite biobricks were used to complement the swimming defect of a motA E. coli mutant.
We found that swimming was restored in the following order:
BBa_J23116 > BBa_J23106 > BBa_J23103 = BBa_J23112.
Examination of the sequences of BBa_J23103 and BBa_J23112 showed that they are identical, despite showing different levels of RFP expression in their initial characterisation!

>BBa_J23103 Part-only sequence (35 bp) ctgatagctagctcagtcctagggattatgctagc

>BBa_J23112 Part-only sequence (35 bp) ctgatagctagctcagtcctagggattatgctagc

Evaluation of Anderson promoter J23103 in B. subtilis by iGEM-Team LMU-Munich 2012

This Anderson promoter was evaluated without fused RFP with the lux operon as a reporter in B. subtilis. See the new BioBrick BBa_K823007 without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in B. subtilis.

User Reviews

UNIQa2e34ee8b2e10afa-partinfo-00000000-QINU

•••••

iGEM HKU 2011

To start characterizing the promoters, we have performed the red florescence intensity measurements for our selected plasmid in the E.Coli MG1655 strain. The data collected is shown below. It is found that promoter J23106 can lead to a higher expression since the fluorescence intensity per OD600 is the highest, while J23103, J23109, J23116 have relative low expression and fluorescence. As our selected promoters have different strength, thus our team is able to use them to fine tune the protein expression.

mRFP fluorescence intensity under different promoters

UNIQa2e34ee8b2e10afa-partinfo-00000002-QINU

@@ Line 4: / Line 4: @@
 ===Applications of BBa_J23103===
+====Use of this promoter by team Glasgow 2014 ====
+[https://parts.igem.org/Part:BBa_J23116 BBa_J23116],
+[https://parts.igem.org/Part:BBa_J23106 BBa_J23106],
+[https://parts.igem.org/Part:BBa_J23103 BBa_J23103], and
+[https://parts.igem.org/Part:BBa_J23112 BBa_J23112]
+were used to express motA and motB together in our composite biobricks: <br>
+[https://parts.igem.org/Part:BBa_K1463773 BBa_ K1463773],
+[https://parts.igem.org/Part:BBa_K1463772 BBa_ K1463772],
+[https://parts.igem.org/Part:BBa_K1463770 BBa_ K1463770], and
+[https://parts.igem.org/Part:BBa_K1463771 BBa_ K1463771] respectively. <br>
+These composite biobricks were used to complement the swimming defect of a <em>motA E. coli</em> mutant.<br>
+We found that swimming was restored in the following order: <br>
+[https://parts.igem.org/Part:BBa_J23116 BBa_J23116] >
+[https://parts.igem.org/Part:BBa_J23106 BBa_J23106] >
+[https://parts.igem.org/Part:BBa_J23103 BBa_J23103] =
+[https://parts.igem.org/Part:BBa_J23112 BBa_J23112]. <br>
+Examination of the sequences of [https://parts.igem.org/Part:BBa_J23103 BBa_J23103]  and [https://parts.igem.org/Part:BBa_J23112 BBa_J23112] showed that they are identical, despite showing different levels of RFP expression in their initial characterisation! <br>
+>BBa_J23103 Part-only sequence (35 bp) ctgatagctagctcagtcctagggattatgctagc
+>BBa_J23112 Part-only sequence (35 bp) ctgatagctagctcagtcctagggattatgctagc
 ====Evaluation of Anderson promoter J23103 in ''B. subtilis'' by iGEM-Team LMU-Munich 2012====
 This Anderson promoter was evaluated without fused RFP with the ''lux'' operon as a reporter in ''B. subtilis''. See the new BioBrick [https://parts.igem.org/Part:BBa_K823007 BBa_K823007] without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in ''B. subtilis''.
 ===User Reviews===