Difference between revisions of "Part:BBa K784047:Design"

 
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===Design Notes===
 
===Design Notes===
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The riboswitch had to fused to the RNAP using assembly PCR.
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The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7 RNAP are adjacent to one another without any restriction site between them. There is an additional stop codon at the end of the gene.
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===Source===
 
===Source===

Latest revision as of 10:40, 26 September 2012

Theophylline riboswitch + wt T7 RNAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RBS and the start codon of the gene are part of the riboswitch sequence and are responsible for its structure, so they are not to be changed. This part was constructed using fusion PCR, so the RS and the T7 RNAP are adjacent to one another without any restriction site between them. There is an additional stop codon at the end of the gene.

Source

Assembly PCR of BBa K784005 and BBa_I2032. BBa I2032 was received from Ann K. Ganesan.

References