Difference between revisions of "Part:BBa K784010"
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This is a construct containing an [[Part:BBa_K784008|MCS followed by a tac promoter]] followed by the [[Part:BBa_K784005|theophylline riboswitch]] fused to mCherry. The construct was created using [http://openwetware.org/wiki/Assembly_pcr Assembly pcr]. This construct was used to characterize the relationship between the theophylline concentration and the level of translation of mCherry fused to the theophylline riboswitch.<br> | This is a construct containing an [[Part:BBa_K784008|MCS followed by a tac promoter]] followed by the [[Part:BBa_K784005|theophylline riboswitch]] fused to mCherry. The construct was created using [http://openwetware.org/wiki/Assembly_pcr Assembly pcr]. This construct was used to characterize the relationship between the theophylline concentration and the level of translation of mCherry fused to the theophylline riboswitch.<br> | ||
| − | The physical DNA of this part has been submitted in pSB1C3 to the registry. However, it hasn't been cloned using the biobrick prefix and suffix. It has been inserted using the EcoRI and PstI restriction sites flanking the insert, destroying the biobrick prefix and suffix. | + | The physical DNA of this part has been submitted in pSB1C3 to the registry. However, it hasn't been cloned using the biobrick prefix and suffix. It has been inserted using the EcoRI and PstI restriction sites flanking the insert, destroying the biobrick prefix and suffix.<br> |
| + | The physical DNA of the riboswitch+mCherry construct has been submitted to the registry in compliance to the RFC10 standard as [https://parts.igem.org/wiki/index.php?title=Part:BBa_K784006 BBa_K784006]. | ||
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Latest revision as of 08:16, 30 September 2012
MCS+tac promoter+theophylline riboswitch+mCherry
This is a construct containing an MCS followed by a tac promoter followed by the theophylline riboswitch fused to mCherry. The construct was created using [http://openwetware.org/wiki/Assembly_pcr Assembly pcr]. This construct was used to characterize the relationship between the theophylline concentration and the level of translation of mCherry fused to the theophylline riboswitch.
The physical DNA of this part has been submitted in pSB1C3 to the registry. However, it hasn't been cloned using the biobrick prefix and suffix. It has been inserted using the EcoRI and PstI restriction sites flanking the insert, destroying the biobrick prefix and suffix.
The physical DNA of the riboswitch+mCherry construct has been submitted to the registry in compliance to the RFC10 standard as BBa_K784006.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]