Difference between revisions of "Part:BBa K299007"
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Latest revision as of 22:34, 25 October 2010
RBS measurement device J23100 B0032 GFP
This part is licensed under
Creative BioCommons
This device was used to determine BBa_B0032 strength relative to BBa_B0034.
Authors
Clonning:
- Ania Puławska
- Ania Safray
- Milena Bażlekowa
The lab work was supervised by:
- Jarek Pankowski
- Ania Olchowik
- Kasia Grześ
Flow cytometer measurements done by Cherry Moreno
Construct design
The device consists of BBa_J23100 promoter followed by BBa_B0032 community RBS and BBa_I13401 reporter module. GFPmut3b was used as reporter protein.
Measurements
Using this construct we have determined that BBa_B0032 strength is 33,96% relative to BBa_B0034
All our measurement results are summarized on the chart below:
For more info please look at Team Warsaw 2010 [http://2010.igem.org/Team:Warsaw/Stage1/RBSMeas RBS measurement page].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 706