Difference between revisions of "Part:BBa K4390058"
 
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'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
 
'''This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard [[Help:Standards/Assembly/Type_IIS|which is also accepted by iGEM.]]'''
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'''This is a level 1 part formed by assembly of the following level 0 parts:'''
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{| class="wikitable" style="margin:auto"
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|-
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| Promoter || [[part:BBa_J23100|J23100]]
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|-
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| RBS || [[part:BBa_B0034|B0034]]
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|-
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| N part || [[part:BBa_K4390016|K4390016]]
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|-
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| O part || [[part:BBa_K4390007|K4390007]]
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|-
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| C part || [[part:BBa_I746916|I746916]]
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|-
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| Terminator || [[part:BBa_K4390001|K4390001]]
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|}
  
 
==Usage and Biology==
 
==Usage and Biology==
−
This part contains sfGFP fused with 6x-his tag. This part is used as control for his-tagged MT transformation and expressions.
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The green fluorescent protein (GFP) is a protein that can produce bright green colour. Due to these properties, it has always been used as reporter of protein expression. Protein fused with sfGFP will turn the colony green if expressed, which can be used to identify the ability of certain genes to be expressed in other organism like E. coli. Superfolder GFP (sfGFP) is a more robustly folded version of GFP, which is derived from GFP that often misfolds when expressed as fusions with other proteins. Superfolder GPF shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics.
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This part contains sfGFP fused with 6x-his tag. This part is used to check whether the assemply is successful, and also act as control for his-tagged MT transformation and expressions.
  
 
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==<span class='h3bb'>Sequence and Features</span>==
 
==<span class='h3bb'>Sequence and Features</span>==
 
<partinfo>BBa_K4390058 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4390058 SequenceAndFeatures</partinfo>

Latest revision as of 23:40, 13 October 2022


His-tagged sfGFP expression construct (JUMP assembled)

This part is not compatible with BioBrick RFC10 assembly but is compatible with the iGEM Type IIS Part standard which is also accepted by iGEM.

This is a level 1 part formed by assembly of the following level 0 parts:

Promoter J23100
RBS B0034
N part K4390016
O part K4390007
C part I746916
Terminator K4390001

Usage and Biology

The green fluorescent protein (GFP) is a protein that can produce bright green colour. Due to these properties, it has always been used as reporter of protein expression. Protein fused with sfGFP will turn the colony green if expressed, which can be used to identify the ability of certain genes to be expressed in other organism like E. coli. Superfolder GFP (sfGFP) is a more robustly folded version of GFP, which is derived from GFP that often misfolds when expressed as fusions with other proteins. Superfolder GPF shows improved tolerance of circular permutation, greater resistance to chemical denaturants and improved folding kinetics.

This part contains sfGFP fused with 6x-his tag. This part is used to check whether the assemply is successful, and also act as control for his-tagged MT transformation and expressions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 128