Difference between revisions of "Part:BBa K157040"

Latest revision as of 03:36, 9 December 2009

Eukaryotic terminator vector (very similar to pSB1AK8)

This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

It is derived from BBa_J52017. There are a couple differences. It lacks the 3' G in the BioBrick prefix (which should be present to comply with the assembly standard 10). It is also missing an A in the BioBrick suffix between the SpeI site and NotI site (which also should be present to comply with assembly standard 10).

RS 19:26, 23 November 2008 (UTC): This part has been split into a plasmid backbone and part and remapped to BBa_K157040-BBa_K157042. BBa_K157040 is the plasmid backbone and BBa_K157042 is the part. The physical DNA needs to be remapped to BBa_K157042.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 8
Illegal NotI site found at 3411
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XhoI site found at 1272
Illegal XhoI site found at 2298
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 16
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3405
Plasmid lacks a suffix.
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2444

@@ Line 4: / Line 4: @@
 This vector is originally BioBrick vector pSB1AK3 with eukaryotic terminator added. pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
-It is derived from [[Part:BBa_J52017|BBa_J52017]].  The only difference is that it is one base pair shorter.  It lacks the 3' G in the BioBrick prefix (which should be present to comply with the BioBrick standard).
+It is derived from [[Part:BBa_J52017|BBa_J52017]].  There are a couple differences.  It lacks the 3' G in the BioBrick prefix (which should be present to comply with the assembly standard 10).  It is also missing an A in the BioBrick suffix between the SpeI site and NotI site (which also should be present to comply with assembly standard 10).
 *'''[[User:Rshetty|RS]] 19:26, 23 November 2008 (UTC)''': This part has been split into a plasmid backbone and part and remapped to BBa_K157040-BBa_K157042.  BBa_K157040 is the plasmid backbone and BBa_K157042 is the part.  The physical DNA needs to be remapped to BBa_K157042.