Difference between revisions of "Part:BBa K3657031"

Latest revision as of 02:19, 28 October 2020

MS2 Coat Protein coupled with C-terminal GFP

This is a fusion protein containing MS2 Coat Protein (BBa_K3218006) and C-terminal Split-GFP (BBa_K3657028).

Usage and Biology

MS2 Coat Protein binds specifically the MS2 RNA hairpin (BBa_K3113006).

C-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of N-terminal Split-GFP (BBa_K3657027).

This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: BBa_K3657036. The part with the N-terminal of the Split-GFP planned for the experiment: BBa_K3657030. You can find out more about the planned experiment here: iGEM Heidelberg 2020.

Due to the COVID19 pandemics we did not obtain empirical data about this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 15: / Line 15: @@
 <p>C-terminal Split-GFP is a protein that combines to a fluorescent complex when in proximity of N-terminal Split-GFP (<a href="https://parts.igem.org/Part:BBa_K3657027">BBa_K3657027</a>).</p>
+<br>
+<p>This construct was planned to be used to explore the ability to use RNA as a linker between proteins. Following linker should be used: <a href="https://parts.igem.org/Part:BBa_K3657036">BBa_K3657036</a>. The part with the N-terminal of the Split-GFP planned for the experiment: <a href="https://parts.igem.org/Part:BBa_K3657030">BBa_K3657030</a>. You can find out more about the planned experiment here: <a href="https://2020.igem.org/Team:Heidelberg/RNA_Protein">iGEM Heidelberg 2020.</a></p>
+<p> Due to the COVID19 pandemics we did not obtain empirical data about this part.</p>
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