Difference between revisions of "Part:BBa K3137002"
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<partinfo>BBa_K3137002 short</partinfo> | <partinfo>BBa_K3137002 short</partinfo> | ||
| − | PputA is the inducible promoter.We screened the whole-genome transcriptome data by setting the conditions that E5_FPKM>E0_FPKM*10 and E0_FPKM < 150, and selected the significant differentially expression genes which express during the early stage of bacteriophage invasion, such as dadA and lldP. We selected the promoter of PputA as our phage-induced promoter in the early stage because the PputA has the maximun value of log2（E5_FPKM/E0_FPKM）,which means the PputA has the most significant differentially expression during the early period. | + | PputA is the inducible promoter.We screened the whole-genome transcriptome data by setting the conditions that E5_FPKM>E0_FPKM*10 and E0_FPKM < 150, and selected the significant differentially expression genes which express during the early stage of bacteriophage invasion, such as <i>dadA</i> and <i>lldP</i>. We selected the promoter of PputA as our phage-induced promoter in the early stage because the PputA has the maximun value of log2（E5_FPKM/E0_FPKM）,which means the PputA has the most significant differentially expression during the early period. |
[[Image:EARLY PROMOTER 2.gif|400X400 px]] | [[Image:EARLY PROMOTER 2.gif|400X400 px]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | PputA is the inducible promoter that When E.coli is infected by a phage, both report and | + | PputA is the inducible promoter that When <i>E.coli</i> is infected by a phage, both report and responce gene circuits (([https://parts.igem.org/Part:BBa_K3137013# BBa_K3137013])([https://parts.igem.org/Part:BBa_K3137014# BBa_K3137014])) are induced to express at the same time. When a phage infects <i>Escherichia coli</i> into the incubation period (about 5 min), PputA will induce the bacteria to express resistant proteins(([https://parts.igem.org/Part:BBa_K3137006# BBa_K3137006])([https://parts.igem.org/Part:BBa_K3137005# BBa_K3137005])) and display green fluorescence. |
| − | [[Image:EARLY PROMOTER.png|400px]] | + | [[Image:EARLY PROMOTER.png|400px|left]] |
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| + | [[Image:PputA.png|500px|thumb|center|Characterization of PputA]] | ||
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| + | <br><br><br><br><br> | ||
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Latest revision as of 20:28, 21 October 2019
PputA
PputA is the inducible promoter.We screened the whole-genome transcriptome data by setting the conditions that E5_FPKM>E0_FPKM*10 and E0_FPKM < 150, and selected the significant differentially expression genes which express during the early stage of bacteriophage invasion, such as dadA and lldP. We selected the promoter of PputA as our phage-induced promoter in the early stage because the PputA has the maximun value of log2（E5_FPKM/E0_FPKM）,which means the PputA has the most significant differentially expression during the early period.
Usage and Biology
PputA is the inducible promoter that When E.coli is infected by a phage, both report and responce gene circuits ((BBa_K3137013)(BBa_K3137014)) are induced to express at the same time. When a phage infects Escherichia coli into the incubation period (about 5 min), PputA will induce the bacteria to express resistant proteins((BBa_K3137006)(BBa_K3137005)) and display green fluorescence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]