Part:BBa_K3137006

abpAB

abpAB are two genes of the E.coli genome that express resistant proteins that are resistant to T2,T4,T7 and λ phages. We obtained abpA and abpB by PCR from the genome of E.coli BL21 and constructed recombinant plasmid that connected abpA and abpB at the same time, then used IPTG to induce the expression of resistant protein and verified the resistant function of the protein.

Usage and Biology

abpAB encodes the protein AbpAB, which can inhibit the replication and late gene expression of phage, resulting in blocking of phage propagation. AbpAB has no effect on the bacterial growth(Fig 1) which is important to the industry. However, protein AbpAB didn’t work well as we expected(Fig 2).

Figure 1. The effect of protein AbpAB on growth of E. coli BL21

Figure 2. E. coli BL21-pET28a（left）and E. coli BL21-pET28a-abpAB(right)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 222
  Illegal EcoRI site found at 990
  Illegal PstI site found at 255
  Illegal PstI site found at 1647
  Illegal PstI site found at 2727
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 222
  Illegal EcoRI site found at 990
  Illegal PstI site found at 255
  Illegal PstI site found at 1647
  Illegal PstI site found at 2727
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 222
  Illegal EcoRI site found at 990
  Illegal BglII site found at 561
  Illegal BglII site found at 1621
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 222
  Illegal EcoRI site found at 990
  Illegal PstI site found at 255
  Illegal PstI site found at 1647
  Illegal PstI site found at 2727
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 222
  Illegal EcoRI site found at 990
  Illegal PstI site found at 255
  Illegal PstI site found at 1647
  Illegal PstI site found at 2727
  Illegal NgoMIV site found at 3333
  
• 1000
  COMPATIBLE WITH RFC[1000]