Part:BBa_K3137013

report circuit

Usage and Biology

The report gene circuit consists of two periods of phage infection. In incubation period (about 5 min), there is an inducible promoter PputA((BBa_K3137002)) and a green fluorescent protein gene gfp(BBa_K3137011). In burst period (about 20 min), there is an inducible promoter PglcF(BBa_K3137003), and a red fluorescent protein gene mCherry(BBa_K3137004).

When E. coli is infected by a phage, report gene circuit is induced to express. When a phage infects E. coli into the incubation period (about 5 min), the inducible promoter PputA(BBa_K3137002) will induce the bacteria to expressthe green fluorescent protein gene gfp(BBa_K3137011).

Green fluorescence of E.coli before and after phage infection

If the resistant proteins successfully resist phage infection, the inducible promoter PglcF(BBa_K3137003) will not induce the expression of downstream gene. If the resistant proteins fail to kill phages, it means that phages will continue to infect bacteria, when it comes to the burst period (around 20 min) of phage infection, the inducible promoter PglcF(BBa_K3137003) will activate the red fluorescent protein gene mCherry. so that the bacteria will display red fluoresce and lyse cells before the assembly of phages.

Red fluorescence of E.coli before and after phage infection

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 700
  Illegal BsaI.rc site found at 1535