Difference between revisions of "Part:BBa K3165037"
 
(5 intermediate revisions by one other user not shown)
Line 3: Line 3:
 
<partinfo>BBa_K3165037 short</partinfo>
 
<partinfo>BBa_K3165037 short</partinfo>
  
−
This part is used as a generator for mini-CcaS under a constitutive promoter and a strong ribosome site with a double terminator.
+
This part is used as a generator for mini-CcaS <html><a  href="https://parts.igem.org/Part:BBa_K3165000">(BBa_K3165000)</a></html> under a constitutive promoter <html><a  href="https://parts.igem.org/Part:BBa_J23106">(BBa_J23106)</a></html> and a strong ribosome site <html><a  href="https://parts.igem.org/Part:BBa_K3165005">(BBa_K3165005)</a></html> with a double terminator <html><a  href="https://parts.igem.org/Part:BBa_B0015">(BBa_B0015)</a></html>.
  
  
Line 12: Line 12:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain. It is the light-sensitive part of the photo-sensitive CcaS-CcaR system. <br>
+
CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain.<br>
 +
It is the light-sensitive part of the photo-sensitive CcaS-CcaR system.  
 
This part helps in the synthesis of CcaS protein constitutively under a strong ribosome binding site. In association with the Ho1-PcyA-CcaR gene system, this part forms the complete optogenetic CcaS-CcaR system.
 
This part helps in the synthesis of CcaS protein constitutively under a strong ribosome binding site. In association with the Ho1-PcyA-CcaR gene system, this part forms the complete optogenetic CcaS-CcaR system.
  

Latest revision as of 11:42, 21 October 2019


mini-CcaS Generator

This part is used as a generator for mini-CcaS (BBa_K3165000) under a constitutive promoter (BBa_J23106) and a strong ribosome site (BBa_K3165005) with a double terminator (BBa_B0015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

CcaS protein is a cyanobacteriochrome sensor histidine kinase consisting of an N-terminal GAF domain (cGMP phosphodiesterase/ adenyl cyclase/ FhlA) bound to two PAS domains of unknown function attached to a C-terminal Histidine Kinase domain.
It is the light-sensitive part of the photo-sensitive CcaS-CcaR system. This part helps in the synthesis of CcaS protein constitutively under a strong ribosome binding site. In association with the Ho1-PcyA-CcaR gene system, this part forms the complete optogenetic CcaS-CcaR system.