Difference between revisions of "Part:BBa K3044023"
 
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<partinfo>BBa_K3044016 short</partinfo>
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<partinfo>BBa_K3044023 short</partinfo>
  
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This dCas9 is a codon optimized version of <partinfo>BBa_K1150000</partinfo> for expression in E. coli. Together with a single guide RNA (sgRNA), the protein can be used for silencing of specific target genes. dCas9 is the inactive form of the endonuclease Cas9. In contrast to Cas9, dCas9 does not cleave the DNA. Instead, it sterically blocks the elongation of transcription as a result of the sgRNA-DNA interaction. This will only repress the expression of the target gene thus avoiding the destruction of the gene. [1]
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This dCas9 is a codon optimized version of <partinfo>BBa_K1150000</partinfo> for expression in E. coli. Together with a single guide RNA (sgRNA), the protein can be used for silencing of specific target genes. dCas9 is the inactive form of the endonuclease Cas9. In contrast to Cas9, dCas9 does not cleave the DNA. Instead, it sterically blocks the elongation of transcription as a result of the sgRNA-DNA interaction. This will only repress the expression of the target gene thus avoiding the destruction of the gene. [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4343198/]
  
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The dCas9 is asembled with the constitutive active promoter <partinfo>BBa_J23100</partinfo>, an RBS <partinfo>BBa_B0034</partinfo> and a terminator <partinfo>BBa_B0010</partinfo>.
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The dCas9 is asembled with the constitutive active promoter <partinfo>BBa_J23100</partinfo>, the RBS <partinfo>BBa_B0034</partinfo> and the terminator <partinfo>BBa_B0010</partinfo>.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3044016 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3044023 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3044016 parameters</partinfo>
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<partinfo>BBa_K3044023 parameters</partinfo>
 
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Latest revision as of 23:58, 7 October 2019


dCas9 with constitutive active promotor

This dCas9 is a codon optimized version of BBa_K1150000 for expression in E. coli. Together with a single guide RNA (sgRNA), the protein can be used for silencing of specific target genes. dCas9 is the inactive form of the endonuclease Cas9. In contrast to Cas9, dCas9 does not cleave the DNA. Instead, it sterically blocks the elongation of transcription as a result of the sgRNA-DNA interaction. This will only repress the expression of the target gene thus avoiding the destruction of the gene. [1]

The dCas9 is asembled with the constitutive active promoter BBa_J23100, the RBS BBa_B0034 and the terminator BBa_B0010.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 312
    Illegal BglII site found at 1386
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]