Difference between revisions of "Part:BBa K2623011"
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The sequence encoding for the gene is preceded by a promoter <partinfo>BBa_J23100</partinfo>, an RBS <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K2623004</partinfo>, <partinfo>BBa_K2623005</partinfo>, <partinfo>BBa_K2623006</partinfo> and followed by a double terminator <partinfo>BBa_B0015</partinfo> in pSB1C3 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA. | The sequence encoding for the gene is preceded by a promoter <partinfo>BBa_J23100</partinfo>, an RBS <partinfo>BBa_B0034</partinfo>, <partinfo>BBa_K2623004</partinfo>, <partinfo>BBa_K2623005</partinfo>, <partinfo>BBa_K2623006</partinfo> and followed by a double terminator <partinfo>BBa_B0015</partinfo> in pSB1C3 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA. | ||
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| − | [[Image:PKaiBC RpaA .gif|thumb|700px|Fig.1 Simplified model of the central circadian oscillator and output activities.]] | + | <table><tr><th>[[Image:PKaiBC RpaA .gif|thumb|700px|Fig.1 Simplified model of the central circadian oscillator and output activities.[1]]]</th><th></table> |
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===Indentification=== | ===Indentification=== | ||
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<br>In order to verify the effectiveness of our Kai system, we verified the Escherichia coli that was transferred into the Kai loop under the fluorescence microscope after synchronization.<br> | <br>In order to verify the effectiveness of our Kai system, we verified the Escherichia coli that was transferred into the Kai loop under the fluorescence microscope after synchronization.<br> | ||
Since the Kai system is designed for periodic output of signals in Escherichia coli, we evaluateD the changes of fluorescent signals over time. Here are the curves we made. More details can be found in http://2018.igem.org/Team:XMU-China/Measurement | Since the Kai system is designed for periodic output of signals in Escherichia coli, we evaluateD the changes of fluorescent signals over time. Here are the curves we made. More details can be found in http://2018.igem.org/Team:XMU-China/Measurement | ||
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<table><tr><th> | <table><tr><th> | ||
[[Image:GroupA.png|thumb|400px|Fig.3 The bioluminescence at different times in GroupA. The negative value is cause by the value of the control group.]]</th><th> | [[Image:GroupA.png|thumb|400px|Fig.3 The bioluminescence at different times in GroupA. The negative value is cause by the value of the control group.]]</th><th> | ||
| − | [[Image:GroupB.png|thumb|400px|Fig.4 The bioluminescence at different times in GroupB. The negative value is cause by the value of the control group.]]</th><th></table> | + | [[Image:GroupB.png|thumb|400px|Fig.4 The bioluminescence at different times in GroupB. The negative value is cause by the value of the control group.]]</th><th></table><br> |
| + | <table><tr><th> | ||
| + | [[Image:Math.png|thumb|400px|Fig.5 The fitting curve of the oscillator in GroupA]]</th><th> | ||
| + | [[Image:Mathb.png|thumb|400px|Fig.6 The fitting curve of the oscillator in GroupB]]</th><th></table> | ||
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| + | ===Reference=== | ||
| + | [1]Espinosa J, Boyd J S, Cantos R, et al. Cross-talk and regulatory interactions between the essential response regulator RpaB and cyanobacterial circadian clock output[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(7):2198-203.<br> | ||
| + | More details can be seen in http://2018.igem.org/Team:XMU-China/Results | ||
Latest revision as of 20:01, 17 October 2018
Phosphorylation pathsway builder of Kai
Summary
The sequence encoding for the gene is preceded by a promoter BBa_J23100, an RBS BBa_B0034, BBa_K2623004, BBa_K2623005, BBa_K2623006 and followed by a double terminator BBa_B0015 in pSB1C3 to pSB1C3 to convert the periodic oscillation of KaiC phosphorylation into the downstream transcriptional oscillation using the signal pathways of CikA, SasA and RpaA.
Indentification
In order to verify the effectiveness of our Kai system, we verified the Escherichia coli that was transferred into the Kai loop under the fluorescence microscope after synchronization.
Since the Kai system is designed for periodic output of signals in Escherichia coli, we evaluateD the changes of fluorescent signals over time. Here are the curves we made. More details can be found in http://2018.igem.org/Team:XMU-China/Measurement
Reference
[1]Espinosa J, Boyd J S, Cantos R, et al. Cross-talk and regulatory interactions between the essential response regulator RpaB and cyanobacterial circadian clock output[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(7):2198-203.
More details can be seen in http://2018.igem.org/Team:XMU-China/Results
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 188
Illegal BglII site found at 3666
Illegal BglII site found at 3735
Illegal BamHI site found at 2248 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]