Difference between revisions of "Part:BBa K2656102"

 
 
(5 intermediate revisions by 2 users not shown)
Line 3: Line 3:
 
<partinfo>BBa_K2656102 short</partinfo>
 
<partinfo>BBa_K2656102 short</partinfo>
  
−
Transcriptional unit created in a Golden Gate reaction using BsaI endonuclease
+
Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.
 +
 
 +
This  composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com '''BioArt DNA toolkit palette: Printone '''], as it can be used to express a green bright color with low intensity.
 +
 
 +
This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:
 +
<html>
 +
<ul>
 +
<li></html>[https://parts.igem.org/Part:BBa_K2656004 BBa_K2656004]: the [https://parts.igem.org/Part:BBa_J23106 J23106] constitutive promoter in its Golden Braid compatible version <html></li>
 +
<li></html>[https://parts.igem.org/Part:BBa_K2656010 BBa_K2656010]: the [https://parts.igem.org/Part:BBa_B0032 B0032]  weak ribosome biding site in its Golden Braid compatible version <html></li>
 +
<li></html> [https://parts.igem.org/Part:BBa_K2656013 BBa_K2656013]: the [https://parts.igem.org/Part:BBa_I746916 BBa_I746916]  sfGFP sequence in its Golden Braid standardized version <html></li>
 +
<li></html> Terminator  [https://parts.igem.org/Part:BBa_K2656026 BBa_K2656026]: the [https://parts.igem.org/Part:BBa_B0015 B0015] transcriptional terminator in its Golden Braid compatible version<html></li>
 +
</ul>
 +
</html>
 +
 
 +
By using this [http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our  [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and the results of the next graphs have been obtained after this [http://2018.igem.org/Team:Valencia_UPV/Modeling#optimization optimization protocol] and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.
 +
 
 +
[[File:T--Valencia_UPV--optimization_exp1_RBS_graphUPV2018.png|900px|thumb|none|alt=RBS experiment 1.|Figure 1. RBS expression experiment with K2656009, K26560010 and K2656011 RBS basic parts]]
 +
 
 +
 
 +
{|class='wikitable'
 +
|colspan=4|Table 1. Optimized parameters for the BBa_K2656010 RBS.
 +
|-
 +
|'''Parameter'''
 +
|'''Value'''
 +
|-
 +
|Translation rate p
 +
|p = 0.01 min-1
 +
|-
 +
|Dilution rate μ
 +
| μ = 0.01641 min-1
 +
|-
 +
| PoI degradation rate
 +
| RBS Experiment 1 dp = 0.0058 min-1
 +
|}
  
  

Latest revision as of 09:32, 16 October 2018


sfGFP transcriptional unit 3

Constitutive expressed transcriptional unit assembled with a one-pot [http://2018.igem.org/Team:Valencia_UPV/Design Level 1] Golden Gate reaction using BsaI type IIS endonuclease.

This composite part of our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection#com BioArt DNA toolkit palette: Printone ], as it can be used to express a green bright color with low intensity.

This transcriptional unit is composed of the following standardized parts from our [http://2018.igem.org/Team:Valencia_UPV/Part_Collection Part Collection]:

By using this [http://2018.igem.org/Team:Valencia_UPV/Experiments#exp_protocol experimental protocol], we have obtained the parameters to valide our [http://2018.igem.org/Team:Valencia_UPV/Modeling#models constitutive model] and the results of the next graphs have been obtained after this [http://2018.igem.org/Team:Valencia_UPV/Modeling#optimization optimization protocol] and decision-making process, in which the optimal parameters have been selected and the constituent model for each objective has been simulated and compared with the experimental data.

RBS experiment 1.
Figure 1. RBS expression experiment with K2656009, K26560010 and K2656011 RBS basic parts


Table 1. Optimized parameters for the BBa_K2656010 RBS.
Parameter Value
Translation rate p p = 0.01 min-1
Dilution rate μ μ = 0.01641 min-1
PoI degradation rate RBS Experiment 1 dp = 0.0058 min-1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 76