Difference between revisions of "Part:BBa I739003"

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===Part Structure===
 
===Part Structure===
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<p>The Biobrick encodes luxR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0062 BBa_C0062]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23100 BBa_J23100] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of luxR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
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<p>The Biobrick encodes LuxR ([https://parts.igem.org/wiki/index.php/Part:BBa_C0062 BBa_C0062]) under control of the constitutive promoter [https://parts.igem.org/wiki/index.php/Part:BBa_J23105 BBa_J23105] followed by the ribosome binding site [https://parts.igem.org/wiki/index.php/Part:BBa_B0034 BBa_B0034]. The transcription of luxR is terminated by the double terminator [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015].</p>
  
 
===Mode of Action===
 
===Mode of Action===
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===Purpose===
 
===Purpose===
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<p>This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project description, this part is also termed ''Part 3''. The constitutively synthesized luxR interacts with .... when complexed with HSL.
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<p>This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. When complexed with HSL, the constitutively synthesized LuxR interacts with the double promoters [https://parts.igem.org/wiki/index.php/Part:BBa_I739104 BBa_I739104] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739105 BBa_I739105] which are parts of composites [https://parts.igem.org/wiki/index.php/Part:BBa_I739006 BBa_I739006] and [https://parts.igem.org/wiki/index.php/Part:BBa_I739007 BBa_I739007] respectively.</p>
  
 
===Testing===
 
===Testing===
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Checked for uniqueness of restriction enzyme cleavage sites:<br>
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<p>Checked for uniqueness of restriction enzyme cleavage sites:<br>
 
Eco: ok<br>
 
Eco: ok<br>
 
Xba: ok<br>
 
Xba: ok<br>
 
Spe: ok<br>
 
Spe: ok<br>
 
Pst: ok<br>
 
Pst: ok<br>
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</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:51, 26 October 2007

Constitutive expression cassette for LuxR (J23100.B0034.C0062.B0015)

Part Structure

The Biobrick encodes LuxR (BBa_C0062) under control of the constitutive promoter BBa_J23105 followed by the ribosome binding site BBa_B0034. The transcription of luxR is terminated by the double terminator BBa_B0015.

Mode of Action

Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL) (e.g. AHL). This complex binds to a palindromic site on the promoter BBa_R0062, increasing the rate of transcription. So far, this LuxI/R system is the best characterized system of all cell-cell signaling systems.

Purpose

This Biobrick was designed for the [http://2007.igem.org/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. When complexed with HSL, the constitutively synthesized LuxR interacts with the double promoters BBa_I739104 and BBa_I739105 which are parts of composites BBa_I739006 and BBa_I739007 respectively.

Testing

Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]