Difference between revisions of "Part:BBa K1587006:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== | ||
− | We used the sequence BBa_R0082 for pompC. | + | We used the sequence [https://parts.igem.org/Part:BBa_R0082 BBa_R0082] for pompC, BBa_R0010 for lacI bindig site, [https://parts.igem.org/Part:BBa_B0030 BBa_B0030] for the RBS and [https://parts.igem.org/Part:BBa_K327018 BBa_K327018] for cI. |
===References=== | ===References=== | ||
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+ | - Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442<br> | ||
+ | - Lee JM, Lee J, Kim T & Lee SK (2013) Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor. PLoS ONE 8: e52382<br> | ||
+ | - Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571<br> |
Latest revision as of 21:03, 18 September 2015
pompC and lacI box regulated cI expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
We used the sequence BBa_R0082 for pompC, BBa_R0010 for lacI bindig site, BBa_B0030 for the RBS and BBa_K327018 for cI.
References
- Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM & Voigt CA (2005) Synthetic biology: Engineering Escherichia coli to see light. Nature 438: 441–442
- Lee JM, Lee J, Kim T & Lee SK (2013) Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor. PLoS ONE 8: e52382
- Gambetta GA & Lagarias JC (2001) Genetic engineering of phytochrome biosynthesis in bacteria. PNAS 98: 10566–10571