Difference between revisions of "Part:BBa J45400"

Latest revision as of 21:33, 16 March 2008

3-methylbutanal generator

The 3-methylbutanal generator (BBa_J45400) catalyzes the conversion of the cellular metabolite leucine to the banana odor precursor 3-methylbutanal. The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (BBa_R0011) and an odor precursor enzyme generator (BBa_J45398). Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.

Usage and Biology

BBa_J45008 encodes a branched-chain amino acid transaminase derived from the BAT2 gene from Saccharomyces cerevisiae. BAT2 catalyzes the first step in isoamyl alcohol biosynthesis: conversion of leucine to α-ketoisocaproate via enzymatic reaction 2.6.1.42. BBa_J45009 encodes a branched-chain amino acid transaminase derived from the THI3 gene from Saccharomyces cerevisiae. THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of α-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1. An alcohol dehydrogenase from the cellular chassis reduces 3-methylbutanal to isoamyl alcohol in an NADH-dependent reaction.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1250
Illegal EcoRI site found at 2151
Illegal EcoRI site found at 2850
Illegal EcoRI site found at 3036
Illegal SpeI site found at 1944
Illegal AgeI site found at 1114
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_J45400 short</partinfo>
-This Isoamyl Alcohol Generating Device converts leucine into 3-methylbutanal (which is then reduced to isoamyl
+The 3-methylbutanal generator (<partinfo>BBa_J45400</partinfo>) catalyzes the conversion of the cellular metabolite leucine to the banana odor precursor 3-methylbutanal.  The biosynthetic device is composed of two transcriptional devices: a constitutive transcription source (<partinfo>BBa_R0011</partinfo>) and an odor precursor enzyme generator (<partinfo>BBa_J45398</partinfo>).  Odor precursor enzyme generators take as input a transcriptional signal and produce as output enzymes that catalyze production of an odor precursor from a cellular metabolite.
-alcohol by NADH).  The two coding regions of BAT2 and THI3 are derived from the S. cervisiae leucine degradation pathway.
+[[Image:BBa J45400.png|center]]
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+<partinfo>BBa_J45008</partinfo> encodes a branched-chain amino acid transaminase derived from the ''BAT2'' gene from ''Saccharomyces cerevisiae''.  BAT2 catalyzes the first step in isoamyl alcohol biosynthesis: conversion of leucine to &alpha;-ketoisocaproate via enzymatic reaction 2.6.1.42.  <partinfo>BBa_J45009</partinfo> encodes a branched-chain amino acid transaminase derived from the ''THI3'' gene from ''Saccharomyces cerevisiae''.  THI3 catalyzes the second step in isoamyl alcohol biosynthesis: conversion of &alpha;-ketoisocaproate to 3-methylbutanal via enzymatic reaction 4.1.1.1.  An alcohol dehydrogenase from the cellular chassis reduces 3-methylbutanal to isoamyl alcohol in an NADH-dependent reaction.
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