Difference between revisions of "Part:BBa J45400:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_J45400=== | ===Applications of BBa_J45400=== | ||
| + | Isoamyl alcohol (banana odor precursor) production. | ||
===User Reviews=== | ===User Reviews=== | ||
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| + | <partinfo>BBa_J45400 AddReview 1</partinfo><br> | ||
| + | <I>[[User:Rshetty|Reshma Shetty]]</I> | ||
| + | |width='60%' valign='top'| | ||
| + | <partinfo>BBa_J45400</partinfo>, when used in the banana odor biosynthetic system <partinfo>BBa_J45900</partinfo>, may not be functional since no isoamyl acetate is detected. | ||
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| + | ==Characterization== | ||
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| + | ===Gas chromatography analysis of banana odor biosynthetic system=== | ||
| + | The banana odor biosynthetic system (<partinfo>BBa_J45900</partinfo>) includes the isoamyl alcohol generator (<partinfo>BBa_J45400</partinfo>). | ||
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| + | {{:IGEM:MIT/2006/Banana odor biosynthetic system gas chromatograms}} | ||
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Latest revision as of 19:44, 8 March 2008
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_J45400
Isoamyl alcohol (banana odor precursor) production.
User Reviews
UNIQde80679fcacb3069-partinfo-00000000-QINU
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• |
BBa_J45400, when used in the banana odor biosynthetic system BBa_J45900, may not be functional since no isoamyl acetate is detected. |
UNIQde80679fcacb3069-partinfo-00000004-QINU
Characterization
Gas chromatography analysis of banana odor biosynthetic system
The banana odor biosynthetic system (BBa_J45900) includes the isoamyl alcohol generator (BBa_J45400).
No isoamyl acetate production from cellular metabolites by banana biosynthetic system
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.
We designed and constructed a system for banana odor production from intracellular metabolites (BBa_J45900). We analyzed cultures with the banana odor biosynthetic system for isoamyl acetate production but did not measure detectable levels of isoamyl acetate by gas chromatography (A). The banana odor generator (BBa_J45200) in the absence of exogenous isoamyl acetate does not produce measurable quantities of isoamyl acetate (B). For reference, we analyzed a sample with 100 ppm pure isoamyl acetate as well (E). Most E. coli strains produce indole. Octyl acetate was used as an internal standard for all samples containing isoamyl acetate.