Difference between revisions of "Part:BBa K1031941:Experience"

 
 
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<partinfo>BBa_K1031941 AddReview 1</partinfo>
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<I>TPR_China iGEM2020</I>
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This part is not such good in our own experiment. However, we changed the reporter from eGFP ([[Part:BBa_K3599016|BBa_K3599016]]) to sfGFP ([[Part:BBa_K3599017|BBa_K3599017]]), and the induction is rescued. The new part we made is [[Part:BBa_K3599034|BBa_K3599034]].
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====The fluorescence of the sensor induced by small molecules====
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Through the corresponding small molecule sensing experiment, except Dmpr sensor, all our sensors have great induction effects on its corresponding aromatic small molecules. Among which NahR-sfGFP was the best! It is 63 times of the negative control. <b>And here we can see that Xyls-sfGFP is 18 times of the Xyls-eGFP</b>. And what surprises us is that Paax sensor also has a good response effect to small molecules, which has not been detected before.<br>
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[[File:T--TPR China--Sensor1.png|600px|thumb|center|The fluorescence of the sensor induced by small molecules]]<br>
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[[File:T--TPR China--Sensor2.png|600px|thumb|center|The fluorescence of the NahR sensor induced by small molecules]]<br>
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====The fluorescence of the sensor induced by PAN====
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And here is the fluorescence of different sensors induced by PAN.This data shows the same result that NahR-sfGFP responded best.
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[[File:T--TPR China--Sensor3.jpg|600px|thumb|center|The fluorescence of the sensor induced by PAN]]<br>
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Latest revision as of 08:53, 27 October 2020


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1031941

User Reviews

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•

TPR_China iGEM2020

This part is not such good in our own experiment. However, we changed the reporter from eGFP (BBa_K3599016) to sfGFP (BBa_K3599017), and the induction is rescued. The new part we made is BBa_K3599034.

The fluorescence of the sensor induced by small molecules

Through the corresponding small molecule sensing experiment, except Dmpr sensor, all our sensors have great induction effects on its corresponding aromatic small molecules. Among which NahR-sfGFP was the best! It is 63 times of the negative control. And here we can see that Xyls-sfGFP is 18 times of the Xyls-eGFP. And what surprises us is that Paax sensor also has a good response effect to small molecules, which has not been detected before.

The fluorescence of the sensor induced by small molecules

The fluorescence of the NahR sensor induced by small molecules

The fluorescence of the sensor induced by PAN

And here is the fluorescence of different sensors induced by PAN.This data shows the same result that NahR-sfGFP responded best.

The fluorescence of the sensor induced by PAN

UNIQ9dfd9cc42bfd0d36-partinfo-00000002-QINU