Difference between revisions of "Part:BBa K801060:Design"

Latest revision as of 16:38, 19 September 2013

(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1683
Illegal SapI.rc site found at 107

Protein data table for BioBrick BBa_K801060 automatically created by the BioBrick-AutoAnnotator version 1.0

Nucleotide sequence in RFC 10: (underlined part encodes the protein)
GTACACAATGCGTCGT ... TTCGAAAAATAA
ORF from nucleotide position 8 to 1705 (excluding stop-codon)

Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)

1
101
201
301
401
501

MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE
FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW
FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL
DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT
ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE
FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKGTGAWSHPQFEK*

Sequence features: (with their position in the amino acid sequence, see the list of supported features)

	RFC25 scar (shown in bold):	556 to 557
	Strep-tag II:	559 to 566

Amino acid composition:

Ala (A)	33 (5.8%)
Arg (R)	25 (4.4%)
Asn (N)	27 (4.8%)
Asp (D)	34 (6.0%)

Cys (C)	4 (0.7%)
Gln (Q)	24 (4.2%)
Glu (E)	48 (8.5%)
Gly (G)	29 (5.1%)

His (H)	18 (3.2%)
Ile (I)	39 (6.9%)
Leu (L)	64 (11.3%)
Lys (K)	36 (6.4%)

Met (M)	16 (2.8%)
Phe (F)	28 (4.9%)
Pro (P)	17 (3.0%)
Ser (S)	33 (5.8%)

Thr (T)	26 (4.6%)
Trp (W)	14 (2.5%)
Tyr (Y)	27 (4.8%)
Val (V)	24 (4.2%)

Amino acid counting

	Total number:	566
	Positively charged (Arg+Lys):	61 (10.8%)
	Negatively charged (Asp+Glu):	82 (14.5%)
	Aromatic (Phe+His+Try+Tyr):	87 (15.4%)

Biochemical parameters

	Atomic composition:	C₃₀₀₂H₄₅₈₆N₇₇₈O₈₆₈S₂₀
	Molecular mass [Da]:	66105.3
	Theoretical pI:	5.38
	Extinction coefficient at 280 nm [M^-1 cm^-1]:	117230 / 117480 (all Cys red/ox)

Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges

Codon usage

	Organism:	E. coli	B. subtilis	S. cerevisiae	A. thaliana	P. patens	Mammals
	Codon quality (CAI):	good (0.71)	good (0.75)	good (0.69)	good (0.78)	excellent (0.80)	good (0.68)

Alignments (obtained from PredictProtein.org)

	SwissProt:	Q8L5K3 (99% identity on 554 AAs), Q93X23 (54% identity on 536 AAs)
	TrEML:	A7BG59 (99% identity on 554 AAs), Q8L5K1 (96% identity on 554 AAs)
	PDB:	1n1z (44% identity on 530 AAs), 1n20 (44% identity on 525 AAs)

Predictions (obtained from PredictProtein.org)

Subcellular Localization (reliability in brackets)

	Archaea:	cytosol (100%)
	Bacteria:	cytosol (95%)
	Eukarya:	nucleus (15%)

Gene Ontology (reliability in brackets)

	Molecular Function Ontology:	GO:0050551 (24%), GO:0034768 (24%)
	Biological Process Ontology:	GO:0071485 (19%), GO:0009611 (18%)

Predicted features:

	Disulfid bridges:	-
	Transmembrane helices:	-

The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation.
If you have any questions, comments or suggestions, please leave us a comment.

Keywords: limonene synthase, limonene

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Biobrick was sequenced

Backbone:

Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:

BioBrick encodes (+)-limonene synthase
UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:

see toxicity assay

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

BioBrick: BBa_I742111

Forward Primer: TUM12-LIMS1-fw-cons: 5'- GGATCCTCTAGAGTACACAATGCGTCGTTCAGCAAAC - 3'
Reverse Primer: TUM12-LIMS1-rv: 5'- GGATCCACCGGTGCCTTTGGTGCCAGGAGA - 3'

Organism:

Gene sequence derived from Citrus limon

References

Literature references:

[http://www.ncbi.nlm.nih.gov/pubmed/12084056Pubmed: Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002: Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.]

Database references:

[http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]
[http://www.uniprot.org/uniprot/Q8L5K3 Uniprot: (R)-limonene synthase 1]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K801060 SequenceAndFeatures</partinfo>
+<html><!--- Please copy this table containing parameters for BBa_K801060 at the end of the parametrs section ahead of the references. ---><style type="text/css">table#AutoAnnotator {border:1px solid black; width:100%; border-collapse:collapse;} th#AutoAnnotatorHeader { border:1px solid black; width:100%; background-color: rgb(221, 221, 221);} td.AutoAnnotator1col { width:100%; border:1px solid black; } span.AutoAnnotatorSequence { font-family:'Courier New', Arial; } td.AutoAnnotatorSeqNum	{ text-align:right; width:2%; } td.AutoAnnotatorSeqSeq { width:98% } td.AutoAnnotatorSeqFeat1 { width:3% } td.AutoAnnotatorSeqFeat2a { width:27% } td.AutoAnnotatorSeqFeat2b { width:97% } td.AutoAnnotatorSeqFeat3 { width:70% } table.AutoAnnotatorNoBorder { border:0px; width:100%; border-collapse:collapse; } table.AutoAnnotatorWithBorder { border:1px solid black; width:100%; border-collapse:collapse; } td.AutoAnnotatorOuterAmino { border:0px solid black; width:20% } td.AutoAnnotatorInnerAmino { border:1px solid black; width:50% } td.AutoAnnotatorAminoCountingOuter { border:1px solid black; width:40%;  } td.AutoAnnotatorBiochemParOuter { border:1px solid black; width:60%; } td.AutoAnnotatorAminoCountingInner1 { width: 7.5% } td.AutoAnnotatorAminoCountingInner2 { width:62.5% } td.AutoAnnotatorAminoCountingInner3 { width:30% } td.AutoAnnotatorBiochemParInner1 { width: 5% } td.AutoAnnotatorBiochemParInner2 { width:55% } td.AutoAnnotatorBiochemParInner3 { width:40% } td.AutoAnnotatorCodonUsage1 { width: 3% } td.AutoAnnotatorCodonUsage2 { width:14.2% } td.AutoAnnotatorCodonUsage3 { width:13.8% } td.AutoAnnotatorAlignment1 { width: 3% } td.AutoAnnotatorAlignment2 { width: 10% } td.AutoAnnotatorAlignment3 { width: 87% } td.AutoAnnotatorLocalizationOuter {border:1px solid black; width:40%} td.AutoAnnotatorGOOuter {border:1px solid black; width:60%} td.AutoAnnotatorLocalization1 { width: 7.5% } td.AutoAnnotatorLocalization2 { width: 22.5% } td.AutoAnnotatorLocalization3 { width: 70% } td.AutoAnnotatorGO1 { width: 5% } td.AutoAnnotatorGO2 { width: 35% } td.AutoAnnotatorGO3 { width: 60% } td.AutoAnnotatorPredFeat1 { width:3% } td.AutoAnnotatorPredFeat2a { width:27% } td.AutoAnnotatorPredFeat3 { width:70% } div.AutoAnnotator_trans { position:absolute; background:rgb(11,140,143); background-color:rgba(11,140,143, 0.8); height:5px; top:100px; } div.AutoAnnotator_sec_helix { position:absolute; background:rgb(102,0,102); background-color:rgba(102,0,102, 0.8); height:5px; top:110px; } div.AutoAnnotator_sec_strand { position:absolute; background:rgb(245,170,26); background-color:rgba(245,170,26, 1); height:5px; top:110px; } div.AutoAnnotator_acc_buried { position:absolute; background:rgb(89,168,15); background-color:rgba(89,168,15, 0.8); height:5px; top:120px; } div.AutoAnnotator_acc_exposed { position:absolute; background:rgb(0, 0, 255); background-color:rgba(0, 0, 255, 0.8); height:5px; top:120px; } div.AutoAnnotator_dis { position:absolute; text-align:center; font-family:Arial,Helvetica,sans-serif; background:rgb(255, 200, 0); background-color:rgba(255, 200, 0, 1); height:16px; width:16px; top:80px; border-radius:50%; } </style><table id="AutoAnnotator"><tr><!-- Time stamp in ms since 1/1/1970 1379608715361 --><th id="AutoAnnotatorHeader" colspan="2">Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K801060">BBa_K801060</a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0</th></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Nucleotide sequence</strong> in <strong>RFC 10</strong>: (underlined part encodes the protein)<br><span class="AutoAnnotatorSequence">&nbsp;GTACACA<u>ATGCGTCGT&nbsp;...&nbsp;TTCGAAAAA</u>TAA</span><br>&nbsp;<strong>ORF</strong> from nucleotide position 8 to 1705 (excluding stop-codon)</td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid sequence:</strong> (RFC 25 scars in shown in bold, other sequence features underlined; both given below)<br><span class="AutoAnnotatorSequence"><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqNum">1&nbsp;<br>101&nbsp;<br>201&nbsp;<br>301&nbsp;<br>401&nbsp;<br>501&nbsp;</td><td class="AutoAnnotatorSeqSeq">MRRSANYQPSIWDHDFLQSLNSNYTDEAYKRRAEELRGKVKIAIKDVIEPLDQLDLIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLE<br>FRLLRQHGYPVSQEVFNGFKDDQGGFICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVPMLEARW<br>FIHIYERREDKNHLLLELAKMEFNTLQAIYQEELKEISGWWKDTGLGEKLSFARNRLVASFLWSMGIAFEPQFAYCRRVLTISIALITVIDDIYDVYGTL<br>DELEIFTDAVERWDINYALKHLPGYMKMCFLALYNFVNEFAYYVLKQQDFDLLLSIKNAWLGLIQAYLVEAKWYHSKYTPKLEEYLENGLVSITGPLIIT<br>ISYLSGTNPIIKKELEFLESNPDIVHWSSKIFRLQDDLGTSSDEIQRGDVPKSIQCYMHETGASEEVARQHIKDMMRQMWKKVNAYTADKDSPLTGTTTE<br>FLLNLVRMSHFMYLHGDGHGVQNQETIDVGFTLLFQPIPLEDKHMAFTASPGTKG<b>TG</b>A<u>WSHPQFEK</u>*</td></tr></table></span></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Sequence features:</strong> (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a">RFC25 scar (shown in bold):&nbsp;</td><td class="AutoAnnotatorSeqFeat3">556 to 557</td></tr><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2a"><i>Strep</i>-tag II:&nbsp;</td><td class="AutoAnnotatorSeqFeat3">559 to 566</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid composition:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Ala (A)</td><td class="AutoAnnotatorInnerAmino">33 (5.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Arg (R)</td><td class="AutoAnnotatorInnerAmino">25 (4.4%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asn (N)</td><td class="AutoAnnotatorInnerAmino">27 (4.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asp (D)</td><td class="AutoAnnotatorInnerAmino">34 (6.0%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Cys (C)</td><td class="AutoAnnotatorInnerAmino">4 (0.7%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gln (Q)</td><td class="AutoAnnotatorInnerAmino">24 (4.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Glu (E)</td><td class="AutoAnnotatorInnerAmino">48 (8.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gly (G)</td><td class="AutoAnnotatorInnerAmino">29 (5.1%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">His (H)</td><td class="AutoAnnotatorInnerAmino">18 (3.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ile (I)</td><td class="AutoAnnotatorInnerAmino">39 (6.9%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Leu (L)</td><td class="AutoAnnotatorInnerAmino">64 (11.3%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Lys (K)</td><td class="AutoAnnotatorInnerAmino">36 (6.4%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Met (M)</td><td class="AutoAnnotatorInnerAmino">16 (2.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Phe (F)</td><td class="AutoAnnotatorInnerAmino">28 (4.9%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Pro (P)</td><td class="AutoAnnotatorInnerAmino">17 (3.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ser (S)</td><td class="AutoAnnotatorInnerAmino">33 (5.8%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Thr (T)</td><td class="AutoAnnotatorInnerAmino">26 (4.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Trp (W)</td><td class="AutoAnnotatorInnerAmino">14 (2.5%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Tyr (Y)</td><td class="AutoAnnotatorInnerAmino">27 (4.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Val (V)</td><td class="AutoAnnotatorInnerAmino">24 (4.2%)</td></tr></table></td></tr></table></td></tr><tr><td class="AutoAnnotatorAminoCountingOuter"><strong>Amino acid counting</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Total number:</td><td class="AutoAnnotatorAminoCountingInner3">566</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Positively charged (Arg+Lys):</td><td class="AutoAnnotatorAminoCountingInner3">61 (10.8%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Negatively charged (Asp+Glu):</td><td class="AutoAnnotatorAminoCountingInner3">82 (14.5%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Aromatic (Phe+His+Try+Tyr):</td><td class="AutoAnnotatorAminoCountingInner3">87 (15.4%)</td></tr></table></td><td class="AutoAnnotatorBiochemParOuter"><strong>Biochemical parameters</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Atomic composition:</td><td class="AutoAnnotatorBiochemParInner3">C<sub>3002</sub>H<sub>4586</sub>N<sub>778</sub>O<sub>868</sub>S<sub>20</sub></td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Molecular mass [Da]:</td><td class="AutoAnnotatorBiochemParInner3">66105.3</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Theoretical pI:</td><td class="AutoAnnotatorBiochemParInner3">5.38</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td class="AutoAnnotatorBiochemParInner3">117230 / 117480 (all Cys red/ox)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges</strong>&nbsp;<input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot()' value='Show'><span id="hydrophobicity_charge_explanation"></span><div id="hydrophobicity_charge_container" style='display:none'><div id="hydrophobicity_charge_placeholder0" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder1" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder2" style="width:100%;height:150px"></div></div></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Codon usage</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Organism:</td><td class="AutoAnnotatorCodonUsage3"><i>E. coli</i></td><td class="AutoAnnotatorCodonUsage3"><i>B. subtilis</i></td><td class="AutoAnnotatorCodonUsage3"><i>S. cerevisiae</i></td><td class="AutoAnnotatorCodonUsage3"><i>A. thaliana</i></td><td class="AutoAnnotatorCodonUsage3"><i>P. patens</i></td><td class="AutoAnnotatorCodonUsage3">Mammals</td></tr><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):</td><td class="AutoAnnotatorCodonUsage3">good (0.71)</td><td class="AutoAnnotatorCodonUsage3">good (0.75)</td><td class="AutoAnnotatorCodonUsage3">good (0.69)</td><td class="AutoAnnotatorCodonUsage3">good (0.78)</td><td class="AutoAnnotatorCodonUsage3">excellent (0.80)</td><td class="AutoAnnotatorCodonUsage3">good (0.68)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Alignments</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">SwissProt:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/Q8L5K3'>Q8L5K3</a> (99% identity on 554 AAs), <a href='http://www.uniprot.org/uniprot/Q93X23'>Q93X23</a> (54% identity on 536 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">TrEML:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/A7BG59'>A7BG59</a> (99% identity on 554 AAs), <a href='http://www.uniprot.org/uniprot/Q8L5K1'>Q8L5K1</a> (96% identity on 554 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">PDB:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=1n1z'>1n1z</a> (44% identity on 530 AAs), <a href='http://www.rcsb.org/pdb/explore/explore.do?structureId=1n20'>1n20</a> (44% identity on 525 AAs)</td></tr></table></td></tr><tr><th id='AutoAnnotatorHeader' colspan="2"><strong>Predictions</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)</th></tr><tr><td class="AutoAnnotatorLocalizationOuter"><strong>Subcellular Localization</strong> (reliability in brackets)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Archaea:</td><td class="AutoAnnotatorLocalization3">cytosol (100%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Bacteria:</td><td class="AutoAnnotatorLocalization3">cytosol (95%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Eukarya:</td><td class="AutoAnnotatorLocalization3">nucleus (15%)</td></tr></table></td><td class="AutoAnnotatorGOOuter"><strong>Gene Ontology</strong> (reliability in brackets)<br><table class="AutoAnnotatorNoBorder"><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Molecular Function Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0050551'>GO:0050551</a> (24%), <a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0034768'>GO:0034768</a> (24%)</td></tr><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Biological Process Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0071485'>GO:0071485</a> (19%), <a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009611'>GO:0009611</a> (18%)</td></tr><tr><td class='AutoAnnotatorGO1'> </td><td class='AutoAnnotatorGO2'> </td><td class='AutoAnnotatorGO3'>&nbsp;</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Predicted features:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Disulfid bridges:</td><td class="AutoAnnotatorPredFeat3">&nbsp;- </td></tr><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Transmembrane helices:</td><td class="AutoAnnotatorPredFeat3">&nbsp;- </td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.<br>If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.</td></tr></table><br><!-- IMPORTANT: DON'T REMOVE THIS LINE, OTHERWISE NOT SUPPORTED FOR IE BEFORE 9 --><!--[if lte IE 8]><script language="javascript" type="text/javascript" src="excanvas.min.js"></script><![endif]--><script type='text/javascript' src='http://code.jquery.com/jquery-1.10.0.min.js'></script><script type='text/javascript' src='http://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script><script>var hydrophobicity_datapoints = [[2.5,-1.22],[3.5,-2.30],[4.5,-1.66],[5.5,-1.46],[6.5,-1.62],[7.5,-2.14],[8.5,-0.54],[9.5,-0.46],[10.5,-0.46],[11.5,-0.78],[12.5,-1.32],[13.5,-1.66],[14.5,-0.72],[15.5,-0.72],[16.5,-0.24],[17.5,1.22],[18.5,-0.04],[19.5,-0.96],[20.5,-0.96],[21.5,-1.06],[22.5,-1.96],[23.5,-1.96],[24.5,-2.50],[25.5,-1.44],[26.5,-1.44],[27.5,-2.08],[28.5,-2.28],[29.5,-2.48],[30.5,-2.48],[31.5,-2.92],[32.5,-2.84],[33.5,-1.18],[34.5,-1.18],[35.5,-1.62],[36.5,-1.70],[37.5,-0.16],[38.5,-1.70],[39.5,0.10],[40.5,0.54],[41.5,2.22],[42.5,0.60],[43.5,0.68],[44.5,0.62],[45.5,1.16],[46.5,-0.44],[47.5,0.02],[48.5,1.48],[49.5,-0.06],[50.5,-1.66],[51.5,-0.20],[52.5,-0.58],[53.5,-0.58],[54.5,1.02],[55.5,1.02],[56.5,-0.44],[57.5,1.02],[58.5,-0.44],[59.5,-2.24],[60.5,-0.78],[61.5,-0.16],[62.5,-0.16],[63.5,0.90],[64.5,1.16],[65.5,-0.50],[66.5,0.14],[67.5,-1.32],[68.5,-1.82],[69.5,-1.88],[70.5,-0.08],[71.5,-1.54],[72.5,-1.54],[73.5,-0.50],[74.5,0.96],[75.5,-0.64],[76.5,-0.44],[77.5,1.16],[78.5,-0.00],[79.5,-1.46],[80.5,-1.46],[81.5,-1.46],[82.5,-3.14],[83.5,-3.58],[84.5,-3.14],[85.5,-3.14],[86.5,-2.62],[87.5,-2.74],[88.5,-2.82],[89.5,-3.26],[90.5,-3.26],[91.5,-2.32],[92.5,-1.68],[93.5,-0.54],[94.5,0.02],[95.5,0.56],[96.5,0.56],[97.5,0.12],[98.5,0.32],[99.5,-0.44],[100.5,0.48],[101.5,0.48],[102.5,0.28],[103.5,-0.98],[104.5,-0.72],[105.5,-1.56],[106.5,-2.58],[107.5,-2.00],[108.5,-0.46],[109.5,0.02],[110.5,-0.60],[111.5,-1.04],[112.5,0.12],[113.5,-0.16],[114.5,-0.70],[115.5,-0.08],[116.5,1.18],[117.5,-0.44],[118.5,-1.70],[119.5,-1.70],[120.5,-2.32],[121.5,-2.96],[122.5,-2.26],[123.5,-1.00],[124.5,0.60],[125.5,1.80],[126.5,1.18],[127.5,0.56],[128.5,0.56],[129.5,-1.12],[130.5,-1.70],[131.5,-0.10],[132.5,1.36],[133.5,0.64],[134.5,2.18],[135.5,1.62],[136.5,0.02],[137.5,-0.38],[138.5,-0.38],[139.5,-1.40],[140.5,-1.02],[141.5,-0.48],[142.5,-0.08],[143.5,-0.62],[144.5,-0.44],[145.5,-0.88],[146.5,-0.88],[147.5,-0.74],[148.5,0.34],[149.5,-0.28],[150.5,-0.28],[151.5,0.24],[152.5,-0.84],[153.5,-1.92],[154.5,-0.66],[155.5,-0.10],[156.5,-0.62],[157.5,-1.22],[158.5,-1.16],[159.5,-0.96],[160.5,-1.60],[161.5,-2.14],[162.5,-0.52],[163.5,0.50],[164.5,0.64],[165.5,1.26],[166.5,1.18],[167.5,-0.36],[168.5,-0.36],[169.5,-1.96],[170.5,-2.50],[171.5,-2.42],[172.5,-0.88],[173.5,-0.70],[174.5,0.84],[175.5,1.90],[176.5,1.90],[177.5,0.36],[178.5,0.16],[179.5,-1.46],[180.5,-2.72],[181.5,-1.66],[182.5,-0.20],[183.5,-1.26],[184.5,0.28],[185.5,0.86],[186.5,1.26],[187.5,-0.14],[188.5,0.38],[189.5,-1.16],[190.5,-0.00],[191.5,-1.08],[192.5,-0.06],[193.5,0.88],[194.5,0.96],[195.5,0.48],[196.5,-0.10],[197.5,-0.66],[198.5,-0.86],[199.5,0.74],[200.5,-0.26],[201.5,1.54],[202.5,1.46],[203.5,0.20],[204.5,-1.60],[205.5,-1.86],[206.5,-3.46],[207.5,-3.90],[208.5,-3.98],[209.5,-3.78],[210.5,-3.52],[211.5,-2.06],[212.5,-0.60],[213.5,0.94],[214.5,0.94],[215.5,2.34],[216.5,1.94],[217.5,0.40],[218.5,0.02],[219.5,0.02],[220.5,-0.18],[221.5,-1.24],[222.5,-0.60],[223.5,-0.22],[224.5,-0.22],[225.5,-0.42],[226.5,1.18],[227.5,1.06],[228.5,-0.40],[229.5,-0.40],[230.5,-1.46],[231.5,-1.60],[232.5,-2.12],[233.5,-2.12],[234.5,-0.52],[235.5,0.02],[236.5,-0.82],[237.5,-0.22],[238.5,0.30],[239.5,-1.38],[240.5,-1.92],[241.5,-1.98],[242.5,-1.88],[243.5,-0.94],[244.5,-0.24],[245.5,-0.24],[246.5,-0.88],[247.5,-0.04],[248.5,-0.96],[249.5,-0.32],[250.5,0.74],[251.5,0.62],[252.5,-0.84],[253.5,-1.58],[254.5,-1.38],[255.5,-0.90],[256.5,0.36],[257.5,0.90],[258.5,2.36],[259.5,2.36],[260.5,1.34],[261.5,0.82],[262.5,1.36],[263.5,0.72],[264.5,0.86],[265.5,1.40],[266.5,2.12],[267.5,1.04],[268.5,0.80],[269.5,-0.80],[270.5,-0.60],[271.5,-0.80],[272.5,-0.36],[273.5,0.46],[274.5,0.26],[275.5,-1.20],[276.5,-0.72],[277.5,0.30],[278.5,-0.34],[279.5,1.46],[280.5,2.20],[281.5,2.26],[282.5,1.86],[283.5,2.76],[284.5,2.76],[285.5,2.78],[286.5,2.72],[287.5,3.26],[288.5,1.80],[289.5,0.20],[290.5,1.24],[291.5,0.14],[292.5,-1.46],[293.5,0.08],[294.5,0.52],[295.5,-0.46],[296.5,-0.34],[297.5,1.12],[298.5,-0.42],[299.5,-0.86],[300.5,-0.02],[301.5,-0.58],[302.5,-0.44],[303.5,0.82],[304.5,1.38],[305.5,-0.08],[306.5,0.98],[307.5,0.92],[308.5,-0.34],[309.5,-1.10],[310.5,-0.58],[311.5,-1.64],[312.5,-1.58],[313.5,-1.58],[314.5,-0.94],[315.5,-0.40],[316.5,1.06],[317.5,-0.62],[318.5,-0.56],[319.5,0.46],[320.5,-0.22],[321.5,-1.06],[322.5,-0.54],[323.5,0.48],[324.5,-1.06],[325.5,-0.36],[326.5,0.22],[327.5,1.04],[328.5,1.42],[329.5,2.56],[330.5,2.94],[331.5,2.18],[332.5,0.92],[333.5,0.72],[334.5,1.20],[335.5,-0.26],[336.5,-0.70],[337.5,0.56],[338.5,0.36],[339.5,-0.74],[340.5,-0.30],[341.5,1.24],[342.5,1.44],[343.5,0.30],[344.5,-0.14],[345.5,-0.58],[346.5,-2.12],[347.5,-2.32],[348.5,-2.24],[349.5,-0.78],[350.5,0.68],[351.5,2.14],[352.5,1.42],[353.5,3.02],[354.5,1.48],[355.5,0.02],[356.5,-0.38],[357.5,-0.40],[358.5,-0.54],[359.5,0.16],[360.5,1.62],[361.5,2.16],[362.5,1.64],[363.5,1.24],[364.5,1.06],[365.5,1.06],[366.5,1.00],[367.5,1.00],[368.5,1.00],[369.5,0.48],[370.5,-0.46],[371.5,-1.56],[372.5,-1.50],[373.5,-2.02],[374.5,-2.02],[375.5,-2.10],[376.5,-1.98],[377.5,-1.66],[378.5,-2.28],[379.5,-0.74],[380.5,-1.18],[381.5,-1.74],[382.5,-1.68],[383.5,-0.14],[384.5,-1.60],[385.5,-1.60],[386.5,-0.98],[387.5,0.04],[388.5,0.12],[389.5,0.66],[390.5,2.26],[391.5,2.20],[392.5,1.36],[393.5,0.20],[394.5,1.12],[395.5,1.12],[396.5,2.16],[397.5,2.10],[398.5,3.32],[399.5,2.40],[400.5,1.24],[401.5,1.10],[402.5,1.08],[403.5,0.10],[404.5,0.12],[405.5,-0.32],[406.5,-1.40],[407.5,-0.34],[408.5,0.64],[409.5,-0.00],[410.5,-0.08],[411.5,-0.46],[412.5,-0.60],[413.5,-2.20],[414.5,-0.86],[415.5,0.68],[416.5,0.68],[417.5,-0.24],[418.5,-0.24],[419.5,-1.12],[420.5,-2.58],[421.5,-0.98],[422.5,0.02],[423.5,0.08],[424.5,0.22],[425.5,0.76],[426.5,-0.30],[427.5,-1.92],[428.5,-0.38],[429.5,0.36],[430.5,-0.38],[431.5,0.54],[432.5,0.62],[433.5,-0.98],[434.5,-2.24],[435.5,-0.58],[436.5,-1.42],[437.5,-0.86],[438.5,-0.32],[439.5,0.22],[440.5,-1.24],[441.5,-1.86],[442.5,-0.82],[443.5,-1.36],[444.5,-2.10],[445.5,-1.48],[446.5,-1.48],[447.5,-1.54],[448.5,-1.16],[449.5,-1.04],[450.5,-1.12],[451.5,0.48],[452.5,-1.06],[453.5,-0.24],[454.5,0.28],[455.5,0.82],[456.5,-0.72],[457.5,-0.72],[458.5,-1.36],[459.5,-1.18],[460.5,-1.20],[461.5,-0.72],[462.5,-0.72],[463.5,-1.28],[464.5,-0.36],[465.5,-0.36],[466.5,-1.10],[467.5,-1.10],[468.5,-1.04],[469.5,-0.98],[470.5,-2.12],[471.5,-1.92],[472.5,-0.84],[473.5,0.18],[474.5,-1.62],[475.5,-1.54],[476.5,-0.46],[477.5,-1.02],[478.5,-2.18],[479.5,-2.06],[480.5,-0.52],[481.5,-1.60],[482.5,-1.06],[483.5,-0.54],[484.5,0.10],[485.5,-0.38],[486.5,-0.38],[487.5,-1.52],[488.5,-1.96],[489.5,-1.98],[490.5,-2.66],[491.5,-1.20],[492.5,-0.56],[493.5,0.06],[494.5,0.08],[495.5,0.26],[496.5,-0.64],[497.5,-1.20],[498.5,-0.56],[499.5,0.34],[500.5,1.24],[501.5,0.68],[502.5,2.14],[503.5,2.42],[504.5,0.76],[505.5,0.38],[506.5,0.92],[507.5,-0.48],[508.5,-0.76],[509.5,0.52],[510.5,-0.12],[511.5,0.80],[512.5,0.80],[513.5,0.16],[514.5,-0.92],[515.5,-0.74],[516.5,-2.14],[517.5,-1.58],[518.5,-0.66],[519.5,-0.66],[520.5,-1.28],[521.5,-1.34],[522.5,-1.96],[523.5,-2.94],[524.5,-1.34],[525.5,-1.34],[526.5,0.20],[527.5,0.82],[528.5,1.52],[529.5,0.48],[530.5,1.94],[531.5,1.86],[532.5,2.50],[533.5,1.24],[534.5,1.06],[535.5,1.20],[536.5,0.12],[537.5,0.32],[538.5,0.32],[539.5,-0.06],[540.5,-1.74],[541.5,-2.06],[542.5,-2.44],[543.5,-1.38],[544.5,-0.12],[545.5,0.52],[546.5,1.52],[547.5,0.98],[548.5,0.30],[549.5,-0.34],[550.5,-0.34],[551.5,-1.48],[552.5,-1.40],[553.5,-1.22],[554.5,-1.22],[555.5,-0.72],[556.5,-0.12],[557.5,-0.20],[558.5,-0.70],[559.5,-0.94],[560.5,-2.00],[561.5,-1.26],[562.5,-1.80],[563.5,-1.94]];var charge_datapoints = [[2.5,0.40],[3.5,0.40],[4.5,0.20],[5.5,0.00],[6.5,0.00],[7.5,0.00],[8.5,0.00],[9.5,0.00],[10.5,-0.20],[11.5,-0.10],[12.5,-0.30],[13.5,-0.30],[14.5,-0.30],[15.5,-0.10],[16.5,-0.20],[17.5,-0.00],[18.5,-0.00],[19.5,-0.00],[20.5,-0.00],[21.5,-0.00],[22.5,-0.00],[23.5,-0.20],[24.5,-0.40],[25.5,-0.40],[26.5,-0.40],[27.5,-0.20],[28.5,0.20],[29.5,0.60],[30.5,0.60],[31.5,0.40],[32.5,0.00],[33.5,-0.20],[34.5,-0.20],[35.5,-0.20],[36.5,0.20],[37.5,0.40],[38.5,0.60],[39.5,0.40],[40.5,0.40],[41.5,0.20],[42.5,0.40],[43.5,0.00],[44.5,0.00],[45.5,0.00],[46.5,-0.20],[47.5,-0.40],[48.5,-0.20],[49.5,-0.40],[50.5,-0.40],[51.5,-0.20],[52.5,-0.40],[53.5,-0.40],[54.5,-0.20],[55.5,-0.40],[56.5,-0.40],[57.5,-0.20],[58.5,-0.20],[59.5,0.00],[60.5,0.20],[61.5,0.20],[62.5,0.20],[63.5,0.20],[64.5,0.10],[65.5,0.30],[66.5,0.30],[67.5,0.10],[68.5,0.10],[69.5,-0.20],[70.5,-0.40],[71.5,-0.20],[72.5,0.00],[73.5,0.00],[74.5,0.20],[75.5,0.20],[76.5,0.00],[77.5,0.00],[78.5,0.00],[79.5,0.00],[80.5,0.00],[81.5,0.00],[82.5,0.20],[83.5,0.00],[84.5,0.00],[85.5,0.00],[86.5,0.00],[87.5,0.00],[88.5,0.40],[89.5,0.20],[90.5,0.20],[91.5,0.20],[92.5,0.00],[93.5,-0.20],[94.5,0.00],[95.5,0.00],[96.5,0.00],[97.5,-0.20],[98.5,-0.20],[99.5,0.00],[100.5,0.00],[101.5,0.00],[102.5,0.40],[103.5,0.40],[104.5,0.30],[105.5,0.30],[106.5,0.30],[107.5,0.10],[108.5,0.10],[109.5,0.00],[110.5,0.00],[111.5,-0.20],[112.5,-0.20],[113.5,-0.20],[114.5,-0.20],[115.5,-0.20],[116.5,0.00],[117.5,0.20],[118.5,0.00],[119.5,-0.20],[120.5,-0.20],[121.5,-0.20],[122.5,-0.40],[123.5,-0.20],[124.5,0.00],[125.5,0.00],[126.5,-0.20],[127.5,-0.40],[128.5,-0.40],[129.5,-0.20],[130.5,-0.20],[131.5,0.00],[132.5,0.20],[133.5,0.20],[134.5,0.00],[135.5,0.10],[136.5,-0.10],[137.5,-0.10],[138.5,-0.10],[139.5,-0.10],[140.5,-0.20],[141.5,0.00],[142.5,0.00],[143.5,-0.20],[144.5,-0.20],[145.5,-0.40],[146.5,-0.40],[147.5,-0.40],[148.5,-0.20],[149.5,-0.40],[150.5,-0.40],[151.5,-0.40],[152.5,-0.40],[153.5,-0.40],[154.5,-0.20],[155.5,0.00],[156.5,0.00],[157.5,0.20],[158.5,0.30],[159.5,0.30],[160.5,0.50],[161.5,0.30],[162.5,0.10],[163.5,-0.00],[164.5,-0.00],[165.5,-0.20],[166.5,0.20],[167.5,0.20],[168.5,0.20],[169.5,-0.00],[170.5,-0.20],[171.5,-0.60],[172.5,-0.60],[173.5,-0.60],[174.5,-0.40],[175.5,-0.20],[176.5,-0.20],[177.5,-0.20],[178.5,-0.20],[179.5,-0.00],[180.5,0.20],[181.5,0.40],[182.5,0.40],[183.5,0.20],[184.5,-0.00],[185.5,-0.20],[186.5,-0.20],[187.5,-0.10],[188.5,0.10],[189.5,0.30],[190.5,0.30],[191.5,0.30],[192.5,0.20],[193.5,0.20],[194.5,-0.20],[195.5,-0.20],[196.5,-0.00],[197.5,-0.00],[198.5,-0.00],[199.5,0.20],[200.5,0.30],[201.5,0.10],[202.5,0.10],[203.5,-0.10],[204.5,0.10],[205.5,0.20],[206.5,-0.00],[207.5,-0.20],[208.5,0.20],[209.5,-0.00],[210.5,-0.10],[211.5,0.10],[212.5,0.30],[213.5,0.10],[214.5,-0.10],[215.5,-0.20],[216.5,-0.20],[217.5,-0.00],[218.5,-0.00],[219.5,-0.00],[220.5,-0.00],[221.5,-0.00],[222.5,-0.20],[223.5,-0.20],[224.5,-0.00],[225.5,-0.00],[226.5,-0.00],[227.5,-0.00],[228.5,-0.00],[229.5,-0.20],[230.5,-0.40],[231.5,-0.40],[232.5,-0.20],[233.5,-0.40],[234.5,-0.20],[235.5,-0.00],[236.5,-0.00],[237.5,-0.20],[238.5,-0.00],[239.5,0.20],[240.5,-0.00],[241.5,-0.00],[242.5,-0.00],[243.5,-0.00],[244.5,-0.20],[245.5,-0.20],[246.5,-0.00],[247.5,-0.00],[248.5,-0.00],[249.5,-0.00],[250.5,0.20],[251.5,0.20],[252.5,0.20],[253.5,0.40],[254.5,0.40],[255.5,0.40],[256.5,0.20],[257.5,0.20],[258.5,-0.00],[259.5,-0.00],[260.5,-0.00],[261.5,-0.00],[262.5,-0.00],[263.5,-0.00],[264.5,-0.00],[265.5,-0.00],[266.5,-0.00],[267.5,-0.20],[268.5,-0.20],[269.5,-0.20],[270.5,-0.20],[271.5,-0.20],[272.5,-0.00],[273.5,-0.00],[274.5,0.20],[275.5,0.40],[276.5,0.40],[277.5,0.40],[278.5,0.40],[279.5,0.20],[280.5,-0.00],[281.5,-0.00],[282.5,-0.00],[283.5,-0.00],[284.5,-0.00],[285.5,-0.00],[286.5,-0.00],[287.5,-0.00],[288.5,-0.20],[289.5,-0.40],[290.5,-0.40],[291.5,-0.40],[292.5,-0.60],[293.5,-0.40],[294.5,-0.20],[295.5,-0.20],[296.5,-0.20],[297.5,-0.00],[298.5,-0.20],[299.5,-0.40],[300.5,-0.40],[301.5,-0.60],[302.5,-0.60],[303.5,-0.40],[304.5,-0.20],[305.5,-0.40],[306.5,-0.20],[307.5,-0.20],[308.5,-0.40],[309.5,-0.20],[310.5,-0.00],[311.5,-0.20],[312.5,-0.20],[313.5,-0.00],[314.5,-0.20],[315.5,-0.20],[316.5,-0.00],[317.5,0.20],[318.5,0.30],[319.5,0.30],[320.5,0.30],[321.5,0.30],[322.5,0.10],[323.5,-0.00],[324.5,0.20],[325.5,0.20],[326.5,0.20],[327.5,0.20],[328.5,0.20],[329.5,-0.00],[330.5,-0.00],[331.5,-0.00],[332.5,-0.00],[333.5,-0.00],[334.5,-0.00],[335.5,-0.00],[336.5,-0.20],[337.5,-0.20],[338.5,-0.20],[339.5,-0.20],[340.5,-0.20],[341.5,-0.00],[342.5,-0.00],[343.5,0.20],[344.5,0.20],[345.5,0.20],[346.5,-0.00],[347.5,-0.00],[348.5,-0.40],[349.5,-0.40],[350.5,-0.40],[351.5,-0.20],[352.5,-0.20],[353.5,-0.00],[354.5,0.20],[355.5,0.20],[356.5,0.20],[357.5,0.20],[358.5,0.20],[359.5,-0.00],[360.5,-0.00],[361.5,-0.00],[362.5,-0.00],[363.5,-0.00],[364.5,-0.00],[365.5,-0.00],[366.5,-0.00],[367.5,-0.20],[368.5,-0.20],[369.5,-0.00],[370.5,-0.00],[371.5,-0.00],[372.5,0.30],[373.5,0.30],[374.5,0.30],[375.5,0.30],[376.5,0.30],[377.5,0.20],[378.5,0.40],[379.5,0.20],[380.5,-0.00],[381.5,-0.20],[382.5,-0.20],[383.5,-0.40],[384.5,-0.60],[385.5,-0.40],[386.5,-0.20],[387.5,-0.20],[388.5,-0.20],[389.5,-0.00],[390.5,-0.00],[391.5,-0.00],[392.5,-0.00],[393.5,-0.00],[394.5,-0.00],[395.5,-0.00],[396.5,-0.00],[397.5,-0.00],[398.5,-0.00],[399.5,-0.00],[400.5,-0.00],[401.5,-0.00],[402.5,-0.00],[403.5,-0.00],[404.5,-0.00],[405.5,-0.00],[406.5,-0.00],[407.5,-0.00],[408.5,-0.00],[409.5,0.20],[410.5,0.40],[411.5,0.20],[412.5,0.20],[413.5,-0.00],[414.5,-0.20],[415.5,-0.40],[416.5,-0.40],[417.5,-0.40],[418.5,-0.20],[419.5,-0.20],[420.5,-0.40],[421.5,-0.20],[422.5,-0.20],[423.5,-0.10],[424.5,-0.10],[425.5,0.10],[426.5,0.10],[427.5,0.30],[428.5,0.20],[429.5,0.20],[430.5,0.40],[431.5,0.40],[432.5,0.20],[433.5,-0.00],[434.5,-0.20],[435.5,-0.40],[436.5,-0.40],[437.5,-0.40],[438.5,-0.20],[439.5,-0.00],[440.5,-0.20],[441.5,-0.40],[442.5,-0.40],[443.5,-0.40],[444.5,-0.20],[445.5,-0.00],[446.5,-0.00],[447.5,-0.00],[448.5,-0.00],[449.5,-0.00],[450.5,-0.00],[451.5,0.20],[452.5,0.20],[453.5,0.20],[454.5,-0.00],[455.5,-0.00],[456.5,0.10],[457.5,-0.10],[458.5,-0.10],[459.5,-0.10],[460.5,-0.10],[461.5,-0.20],[462.5,-0.20],[463.5,-0.40],[464.5,-0.40],[465.5,-0.40],[466.5,-0.20],[467.5,-0.00],[468.5,0.30],[469.5,0.30],[470.5,0.50],[471.5,0.10],[472.5,0.10],[473.5,-0.00],[474.5,0.20],[475.5,-0.00],[476.5,0.20],[477.5,0.20],[478.5,0.40],[479.5,0.40],[480.5,0.40],[481.5,0.40],[482.5,0.40],[483.5,0.20],[484.5,-0.00],[485.5,-0.00],[486.5,-0.20],[487.5,-0.00],[488.5,-0.20],[489.5,-0.20],[490.5,-0.20],[491.5,-0.00],[492.5,-0.20],[493.5,-0.00],[494.5,-0.00],[495.5,-0.00],[496.5,-0.00],[497.5,-0.20],[498.5,-0.20],[499.5,-0.20],[500.5,-0.20],[501.5,-0.20],[502.5,-0.00],[503.5,-0.00],[504.5,0.20],[505.5,0.20],[506.5,0.20],[507.5,0.30],[508.5,0.30],[509.5,0.10],[510.5,0.10],[511.5,0.10],[512.5,0.10],[513.5,0.10],[514.5,-0.10],[515.5,-0.10],[516.5,-0.00],[517.5,-0.10],[518.5,-0.10],[519.5,0.10],[520.5,0.10],[521.5,-0.00],[522.5,-0.20],[523.5,-0.20],[524.5,-0.20],[525.5,-0.40],[526.5,-0.40],[527.5,-0.20],[528.5,-0.20],[529.5,-0.20],[530.5,-0.00],[531.5,-0.00],[532.5,-0.00],[533.5,-0.00],[534.5,-0.00],[535.5,-0.00],[536.5,-0.00],[537.5,-0.00],[538.5,-0.20],[539.5,-0.40],[540.5,-0.20],[541.5,-0.10],[542.5,-0.10],[543.5,0.10],[544.5,0.30],[545.5,0.10],[546.5,-0.00],[547.5,-0.00],[548.5,-0.00],[549.5,-0.00],[550.5,-0.00],[551.5,0.20],[552.5,0.20],[553.5,0.20],[554.5,0.20],[555.5,0.20],[556.5,-0.00],[557.5,-0.00],[558.5,0.10],[559.5,0.10],[560.5,0.10],[561.5,0.10],[562.5,-0.10],[563.5,-0.00]];var dis_datapoints = [];var trans_datapoints = [];var dis_datapoints = [[14,18],[25,47],[50,63],[66,84],[94,106],[113,120],[128,143],[148,167],[176,185],[195,200],[201,206],[213,221],[223,243],[252,267],[274,295],[300,313],[317,321],[323,347],[352,375],[382,400],[401,405],[412,419],[423,439],[442,446],[452,458],[465,488],[496,512],[524,535]];var dis_datapoints = [[513,514]];var dis_datapoints = [[1,4],[6,6],[15,15],[18,18],[21,23],[25,27],[30,31],[34,35],[38,38],[41,42],[45,46],[49,49],[52,52],[71,71],[75,75],[82,83],[85,86],[88,88],[90,92],[117,117],[120,123],[129,129],[132,132],[144,144],[146,146],[149,149],[156,156],[160,160],[163,164],[167,170],[172,173],[179,179],[182,183],[186,186],[194,194],[206,211],[220,220],[232,232],[235,235],[239,239],[242,243],[245,245],[247,249],[301,301],[308,308],[311,312],[314,314],[316,317],[320,320],[324,324],[338,338],[342,342],[346,349],[351,351],[354,354],[358,358],[362,362],[376,377],[381,381],[383,384],[387,387],[409,409],[412,413],[416,416],[419,420],[423,423],[439,440],[442,444],[446,449],[460,460],[462,462],[464,464],[466,466],[470,470],[474,474],[478,478],[481,481],[485,485],[489,493],[524,525],[528,528],[541,544],[546,546],[548,548],[550,557],[560,560],[562,563],[565,566]];var dis_datapoints = [[9,9],[11,12],[16,17],[33,33],[36,36],[40,40],[43,44],[47,47],[50,51],[54,54],[56,57],[59,61],[63,66],[69,69],[72,73],[76,77],[80,81],[94,99],[101,101],[103,109],[111,113],[115,116],[119,119],[124,124],[126,128],[130,131],[133,143],[145,145],[150,151],[154,155],[157,158],[161,162],[166,166],[177,178],[181,181],[184,185],[187,191],[193,193],[195,196],[198,200],[201,202],[204,205],[215,216],[218,219],[221,223],[225,227],[229,230],[233,234],[237,238],[240,241],[250,250],[252,255],[257,271],[273,274],[276,281],[283,299],[303,303],[306,307],[309,310],[313,313],[318,318],[322,322],[325,326],[328,334],[336,337],[340,341],[344,344],[352,353],[356,356],[359,360],[363,364],[366,368],[370,371],[373,374],[380,380],[382,382],[385,386],[389,400],[401,407],[410,411],[415,415],[418,418],[424,425],[427,435],[438,438],[450,450],[453,458],[468,468],[472,472],[475,476],[479,480],[483,484],[487,488],[498,499],[501,513],[518,518],[526,526],[530,531],[533,535],[537,538],[540,540],[564,564]];var flot_plot_options = []; flot_plot_options[0] = {grid: {borderWidth: {top: 0,right: 0,bottom: 0,left: 0}},legend: {show: false},xaxes: [{show: true,min: 0,max: 200,ticks: [[0.5, '1'], [24.5, '25'], [49.5, '50'], [74.5, '75'], [99.5, '100'], [124.5, '125'], [149.5, '150'], [174.5, '175'], [199.5, '200']],tickLength: -5}],yaxes: [{show: true,ticks: [[0, '0'], [4.5,'hydro-<br>phobic&nbsp;&nbsp;'], [-4.5,'hydro-<br>philic&nbsp;&nbsp;']],min: -4.5,max: +4.5,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(100,149,237,1)'}},{show: true,ticks: [[0, ''], [1,'positive<br>&nbsp;charge'], [-1,'negative<br>&nbsp;charge']],position: 'right',min: -1,max: 1,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(255,99,71,1)'}}]};var number_of_plots = 3;for ( plot_num = 1 ; plot_num < number_of_plots ; plot_num ++){flot_plot_options[plot_num] = $.extend(true, {} ,flot_plot_options[0]);flot_plot_options[plot_num].xaxes = [{min: plot_num*200,max: (plot_num + 1)*200,ticks: [ [plot_num*200 +   0.5, (plot_num*200 +   1).toString()], [plot_num*200 +  24.5, (plot_num*200 +  25).toString()], [plot_num*200 +  49.5, (plot_num*200 +  50).toString()], [plot_num*200 +  74.5, (plot_num*200 +  75).toString()], [plot_num*200 +  99.5, (plot_num*200 + 100).toString()], [plot_num*200 + 124.5, (plot_num*200 + 125).toString()], [plot_num*200 + 149.5, (plot_num*200 + 150).toString()], [plot_num*200 + 174.5, (plot_num*200 + 175).toString()], [plot_num*200 + 199.5, (plot_num*200 + 200).toString()] ],tickLength: -5}];};function show_or_hide_plot(){try {if( $('#hydrophobicity_charge_button').val() =='Show' ){$('#hydrophobicity_charge_container').css('display','block');$('#hydrophobicity_charge_button').val('Hide');var description_html = '<div id='AutoAnnotator_plot_selectors'';description_html = description_html + '<br>&nbsp;<input type='checkbox' id='hydrophobicity_checkbox' checked='checked'>&nbsp;Moving average over 5 amino acids for hydrophobicity (<img src='https://static.igem.org/mediawiki/2013/e/e9/TUM13_hydrophobicity_icon.png' alt='blue graph' height='10'></img>)';description_html = description_html + '<br>&nbsp;<input type='checkbox' id='charge_checkbox' checked='checked'>&nbsp;Moving average over 5 amino acids for charge (<img src='https://static.igem.org/mediawiki/2013/3/3e/TUM13_charge_icon.png' alt='red graph' height='10'></img>)';description_html = description_html + '<br>&nbsp;<input type='checkbox' id='dis_checkbox' checked='checked'>&nbsp;Predicted disulfid bridges (<img src='https://static.igem.org/mediawiki/2013/2/28/TUM13_dis_icon.png' alt='yellow circle' height='10'></img>) with the number of the bridge in the center';description_html = description_html + '<br>&nbsp;<input type='checkbox' id='trans_checkbox' checked='checked'>&nbsp;Predicted transmembrane helices (<img src='https://static.igem.org/mediawiki/2013/7/78/TUM13_trans_icon.png' alt='turquois bars' height='10'></img>)';description_html = description_html + '<br>&nbsp;<input type='checkbox' id='sec_checkbox' checked='checked'>&nbsp;Predicted secondary structure: Helices (<img src='https://static.igem.org/mediawiki/2013/b/bf/TUM13_helix_icon.png' alt='violet bars' height='10'></img>) and beta-strands (<img src='https://static.igem.org/mediawiki/2013/b/bf/TUM13_strand_icon.png' alt='yellow bars' height='10'></img>)';description_html = description_html + '<br>&nbsp;<input type='checkbox' id='acc_checkbox' checked='checked'>&nbsp;Predicted solvent accessability: Exposed (<img src='https://static.igem.org/mediawiki/2013/1/16/TUM13_exposed_icon.png' alt='blue bars' height='10'></img>) and buried (<img src='https://static.igem.org/mediawiki/2013/0/0b/TUM13_buried_icon.png' alt='green bars' height='10'></img>) residues';description_html = description_html + '<br></div>';$('#hydrophobicity_charge_explanation').html(description_html);plot_according_to_selectors();$('#AutoAnnotator_plot_selectors').find('input').click(plot_according_to_selectors);}else{$('#hydrophobicity_charge_container').css('display','none');$('#hydrophobicity_charge_button').val('Show');$('#hydrophobicity_charge_explanation').html('');}}catch(err){txt='There was an error with the button controlling the visibility of the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';alert(txt);}};function plot_according_to_selectors(){try{var plot_datasets = [[],[]];if($('#hydrophobicity_checkbox').prop('checked') == true){plot_datasets[0] = { color: 'rgba(100,149,237,1)',data: hydrophobicity_datapoints,label: 'Hydrophobicity',lines: { show: true, fill: true, fillColor: 'rgba(100,149,237,0.1)' },yaxis: 1};}if($('#charge_checkbox').prop('checked') == true){plot_datasets[1] = {color: 'rgba(255,99,71,1)',data: charge_datapoints,label: 'Charge',lines: { show: true, fill: true, fillColor: 'rgba(255,99,71,0.1)' },yaxis: 2};}for (plot_num = 0 ; plot_num < number_of_plots ; plot_num ++){$.plot('#hydrophobicity_charge_placeholder'+ plot_num.toString(), plot_datasets, flot_plot_options[plot_num] );}var screen_width = $('canvas.flot-base').width(); var pos_of_first_tick = 46;var pos_of_last_tick = screen_width - 51;var tick_diff = (screen_width - 97)/199;if($('#dis_checkbox').prop('checked') == true){for ( j = 0 ; j < dis_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][0] - 1)/200) ).append('<div class='AutoAnnotator_dis' style='left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][0] - 1)*tick_diff - Math.floor((dis_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;'><b>' + (j+1) + '</b></div>');$('#hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][1] - 1)/200) ).append('<div class='AutoAnnotator_dis' style='left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][1] - 1)*tick_diff - Math.floor((dis_datapoints[j][1] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;'><b>' + (j+1) + '</b></div>');}}if($('#trans_checkbox').prop('checked') == true){for ( j = 0 ; j < trans_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((trans_datapoints[j][0] - 1)/200) ).append('<div class='AutoAnnotator_trans' style='width:' + (((trans_datapoints[j][1] - trans_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px ;left:' + ((pos_of_first_tick + (trans_datapoints[j][0] - 1.5)*tick_diff - Math.floor((trans_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px'></div>');}}if($('#sec_checkbox').prop('checked') == true){for ( j = 0 ; j < sec_helix_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((sec_helix_datapoints[j][0] - 1)/200) ).append('<div class='AutoAnnotator_sec_helix' style='width:' + (((sec_helix_datapoints[j][1] - sec_helix_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_helix_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_helix_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px'></div>');}for ( j = 0 ; j < sec_strand_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((sec_strand_datapoints[j][0] - 1)/200) ).append('<div class='AutoAnnotator_sec_strand' style='width:' + (((sec_strand_datapoints[j][1] - sec_strand_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_strand_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_strand_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px'></div>');}}if($('#acc_checkbox').prop('checked') == true){for ( j = 0 ; j < acc_buried_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((acc_buried_datapoints[j][0] - 1)/200) ).append('<div class='AutoAnnotator_acc_buried' style='width:' + (((acc_buried_datapoints[j][1] - acc_buried_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_buried_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_buried_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px'></div>');}for ( j = 0 ; j < acc_exposed_datapoints.length ; j++ ){$('#hydrophobicity_charge_placeholder' + Math.floor((acc_exposed_datapoints[j][0] - 1)/200) ).append('<div class='AutoAnnotator_acc_exposed' style='width:' + (((acc_exposed_datapoints[j][1] - acc_exposed_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_exposed_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_exposed_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px'></div>');}}}catch(err){txt='There was an error while drawing the selected elements for the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';throw(txt);}}</script></html>
 <br>'''Keywords:'''
@@ Line 32: / Line 35: @@
 '''Quality control measures:'''<br>
 * Biobrick was sequenced
 <!--*Test digestion using ?enzyme1? & ?enzyme2?/Not yet performed-->
 <!--*Sequencing using primer ?primer_name?/Not yet sequenced-->
@@ Line 51: / Line 53: @@
 '''Cytotoxicity:'''<br>
+* see toxicity assay
 <!--none/not known/cytotoxic for ''organism name''-->
@@ Line 90: / Line 93: @@
 '''Literature references:'''<br>
-*[[http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002]] Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71.
+*[http://www.ncbi.nlm.nih.gov/pubmed/12084056'''Pubmed:''' Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A., 2002:  Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71.]
-<!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->
 '''Database references:'''<br>
 *[http://www.ncbi.nlm.nih.gov/nuccore/21435702 '''GenBank''': source of the sequence]
 <!--*[http://www.ebi.ac.uk/interpro/IEntry?ac=?accessNr? '''Interpro''': ?title?]-->
-<!--*[http://www.uniprot.org/uniprot/?accessNr? '''Uniprot''': ?title?]-->
+*[http://www.uniprot.org/uniprot/Q8L5K3 '''Uniprot''': (R)-limonene synthase 1]
 <!--*[http://pfam.sanger.ac.uk/family/?accessNr? '''Pfam:''' ?title?]-->
 <!--*[http://www.rcsb.org/pdb/explore/explore.do?structureId=?accessNR? '''PDB:''' ?tile?]-->