Difference between revisions of "Part:pSB1AK3"

Latest revision as of 15:53, 26 February 2009

High copy BioBrick assembly plasmid

pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance. The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).

Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for pSB1AK3

Plasmid map for pSB1AK3

Usage and Biology

Kanamycin resistant up to 5000 ug/ml.

Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)

Primers for these Biobrick vectors can be found in Part:BBa_G00100 (aka VF2) and Part:BBa_G00101 (aka. VR). See also Part:BBa_J18907 (aka VR2) for lower temperature sequencing reactions.

This vector is also available in the Fusion (Freiburg) Biobrick format: Part:BBa_J18902

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 3174
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3168
Illegal XhoI site found at 1035
Illegal XhoI site found at 2061
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 3168
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 3168
Plasmid lacks a suffix.
Illegal XbaI site found at 3183
Illegal SpeI site found at 2
Illegal PstI site found at 16
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2207

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>pSB1AK3 short</partinfo>
 pSB1AK3 is a high copy number plasmid carrying ampicillin and kanamycin resistance.   The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
+Annotated [http://www.biology.utah.edu/jorgensen/wayned/ape/ APE] file for [https://static.igem.org/mediawiki/parts/0/03/PsB1AK3.ogg pSB1AK3] [[Image:PsB1AK3 map.jpg|thumb|center|Plasmid map for pSB1AK3]]
 ===Usage and Biology===
@@ Line 11: / Line 10: @@
 Tetracycline sensitive at 1.5 ug/ml Chloramphicol sensitive at 3.5 ug/ml (spayne and tk 6/6/05)
+*Primers for these Biobrick vectors can be found in [[Part:BBa_G00100]] (aka VF2) and [[Part:BBa_G00101]] (aka. VR). See also [[Part:BBa_J18907]] (aka VR2) for lower temperature sequencing reactions.
+*This vector is also available in the Fusion (Freiburg) Biobrick format: [[Part:BBa_J18902]]