Difference between revisions of "Help:3A Assembly Kit"
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<li>check the contents of the box with the <a href="https://parts.igem.org/Help:3A_Assembly_Kit/Inventory">inventory</a>, to make sure you have everything</li> | <li>check the contents of the box with the <a href="https://parts.igem.org/Help:3A_Assembly_Kit/Inventory">inventory</a>, to make sure you have everything</li> | ||
<li>inspect the materials to make sure nothing has been damaged during shipping</li> | <li>inspect the materials to make sure nothing has been damaged during shipping</li> | ||
| − | <li>refrigerate the agar plates and any materials labeled as requiring storage at 4C.</li> | + | <li>refrigerate the agar plates and any materials labeled as requiring storage at 4C. </li> |
| − | <li>order | + | <li>order an NEB enzyme kit. Click <a href="https://www.neb.com/products/E0546-BioBrick-Assembly-Kit">here</a> for details. |
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<br> | <br> | ||
| − | + | You can download a <a href="https://static.igem.org/mediawiki/parts/e/e9/3A-Assembly-Kit-HiRes.png">hi-res png</a> or <a href="https://static.igem.org/mediawiki/parts/0/0e/3A-Assembly-Kit.pdf">pdf</a> of the images in the protocol. | |
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===Introduction=== | ===Introduction=== | ||
| − | The 3A Assembly Kit and following protocols will take you through the process of 3A Assembly, and by the end you will have assembled your own composite part in the lab. The kit includes two parts: '''Part A''' (<partinfo>BBa_J04500</partinfo>) and '''Part B''' (<partinfo>BBa_J04650</partinfo>), which when assembled together will form a RFP (red fluorescent protein) generator. Your cells will turn <span style="color: red; font-weight: bold;">red!</span> | + | The 3A Assembly Kit and following protocols will take you through the process of [[Help:Assembly#3A_Assembly | 3A Assembly]], and by the end you will have assembled your own composite part in the lab. The kit includes two parts: '''Part A''' (<partinfo>BBa_J04500</partinfo>) and '''Part B''' (<partinfo>BBa_J04650</partinfo>), which when assembled together will form a RFP (red fluorescent protein) generator. Your cells will turn <span style="color: red; font-weight: bold;">red!</span> |
'''Note:''' The 3A Assembly Kit also includes prepared plasmid DNA for '''Part A''' and '''Part B''', so you can skip directly to '''Step #3: Restriction Digest''' if you'd like. But don't forget to take a look at '''Step #1: Grow,''' as you'll need to follow the instructions for growing up cells for '''Step #5: Transformation'''. | '''Note:''' The 3A Assembly Kit also includes prepared plasmid DNA for '''Part A''' and '''Part B''', so you can skip directly to '''Step #3: Restriction Digest''' if you'd like. But don't forget to take a look at '''Step #1: Grow,''' as you'll need to follow the instructions for growing up cells for '''Step #5: Transformation'''. | ||
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===Protocols=== | ===Protocols=== | ||
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Latest revision as of 16:51, 1 June 2017
- Registry Help Pages:
- TOC
- At-a-Glance
- FAQ
Reason: We are no longer providing the 3A Assembly Kit (as of the 2015 season).
| The 3A Assembly Kit |
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Hi High Schoolers,
You've just received the 3A Assembly Kit! We hope your team will enjoy using the kit and in the process learn about synthetic biology based on standard parts and 3A assembly. Before you start please make sure to...
You can download a hi-res png or pdf of the images in the protocol. |
Introduction
The 3A Assembly Kit and following protocols will take you through the process of 3A Assembly, and by the end you will have assembled your own composite part in the lab. The kit includes two parts: Part A (BBa_J04500) and Part B (BBa_J04650), which when assembled together will form a RFP (red fluorescent protein) generator. Your cells will turn red!
Note: The 3A Assembly Kit also includes prepared plasmid DNA for Part A and Part B, so you can skip directly to Step #3: Restriction Digest if you'd like. But don't forget to take a look at Step #1: Grow, as you'll need to follow the instructions for growing up cells for Step #5: Transformation.
Protocols
| Step #1: Growing | |
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Grow up the E. coli that contains your parts! |
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| Step #2: Miniprep | |
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Miniprep cell cultures to extract the DNA for your parts. |
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| Step #3: Restriction Digest | |
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Cut the DNA for your parts and the pSB1C3 linearized plasmid backbone in a restriction digest |
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| Step #4: Ligation | |
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Ligate your two parts together into the pSB1C3 linearized backbone |
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| Step #5: Transformation | |
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Transform the ligation into E. coli to get your newly assembled composite part. |
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| Post your Results! | |
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Post your results! Hopefully you've learned quite a bit while using the 3A Assembly Kit. |
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